PE Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)

CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

10F.9G2

Cat no : PE-65073

Synonyms

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using PE-65073

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2) (PE-65073, Clone: 10F.9G2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65073 targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD274 antigen
• GenBank Accession Number: BC066841
• Gene Symbol: PD-L1
• Gene ID (NCBI): 60533
• RRID: AB_2883870
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

Species	Application	Title
	FC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang
mouse	FC	Oncoimmunology; FXR agonist GW4064 enhances anti-PD-L1 immunotherapy in colorectal cancer; Authors - Lu Lu