Tested Applications
| Positive FC detected in | LPS treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 3 publications below |
| FC | See 13 publications below |
Product Information
PE-65068 targets CD86 in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
LPS-activated CBA/Ca mouse spleen B cells Predict reactive species |
| Full Name | CD86 antigen |
| GenBank Accession Number | BC013807 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 12524 |
| RRID | AB_2883865 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P42082 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells | ||
ACS Appl Mater Interfaces Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy | ||
Int J Nanomedicine Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages | ||
Biochem Biophys Res Commun LILRB4 specific overexpression in myeloid cells promotes tumor progression and immunosuppression in mouse models | ||
Pharmaceutics Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination | ||
J Cardiovasc Transl Res Drp1 Promotes Macrophage M1 Polarization and Inflammatory Response in Autoimmune Myocarditis by Driving Mitochondrial Fission |
