PE Anti-Mouse CD86 (GL1)

CD86 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa


Mouse and More (1)




PE Fluorescent Dye



Cat no : PE-65068


B7, B7 2, B7.2, B70, Cd28l2, Cd86, CD86 antigen, CLS1, ETC 1, Ly 58, Ly58, MB7, MB7 2, TS/A 2

Tested Applications

Positive FC detected inLPS treated mouse splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 6 publications below

Product Information

PE-65068 targets CD86 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen LPS-activated CBA/Ca mouse spleen B cells
Full Name CD86 antigen
GenBank Accession NumberBC013807
Gene Symbol Cd86
Gene ID (NCBI) 12524
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)


Product Specific Protocols
FC protocol for PE CD86 antibody PE-65068Download protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu


The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Authors - Chuyu Xi


Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination

Authors - Xiaojin Chen

J Mater Chem B

PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer

Authors - Lian Zhu

ACS Nano

Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity

Authors - Yunkai Shang

Acta Biomater

Injectable microenvironment-responsive hydrogels with redox-activatable supramolecular prodrugs mediate ferroptosis-immunotherapy for postoperative tumor treatment

Authors - Zhuo Cheng