PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

3

CloneNo.

RB6-8C5

Cat no : PE-65140

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using PE-65140

  1X10^6 mouse bone marrow cells were surface stained with 0.06 ug PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) (PE-65140, Clone: RB6-8C5). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using PE-65140

  1X10^6 mouse bone marrow cells were surface stained with 0.06 ug PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) (PE-65140, Clone: RB6-8C5). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

PE-65140 targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Raised against granulocytes of mouse origin
• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly6g
• Gene ID (NCBI): 546644
• RRID: AB_2883922
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publications

Species	Application	Title
		Int J Mol Med; FTY720 attenuates APAP‑induced liver injury via the JAK2/STAT3 signaling pathway.; Authors - Xiangmin He
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang
		Int J Nanomedicine; Multi-Site Attack, Neutrophil Membrane-Camouflaged Nanomedicine with High Drug Loading for Enhanced Cancer Therapy and Metastasis Inhibition; Authors - Ran Huang