PE Anti-Human CD11b (ICRF44)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

ICRF44

Cat no : PE-65116

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

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Validation Data Gallery

• 

  FC experiment of human PBMCs using PE-65116

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD11b (PE-65116, Clone:ICRF44), or PE Mouse IgG1 Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65116

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD11b (PE-65116, Clone:ICRF44), or PE Mouse IgG1 Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65116 targets CD11b in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 1152 aa, 127 kDa
• GenBank Accession Number: BC096346
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• RRID: AB_2883905
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
human	FC	Nat Commun; Necroptosis enhances 'don't eat me' signal and induces macrophage extracellular traps to promote pancreatic cancer liver metastasis; Authors - Cheng-Yu Liao
human	FC	Eur J Med Chem; Discovery of novel pyrrolo[2,3-d]pyrimidines as potent menin-mixed lineage leukemia interaction inhibitors; Authors - Huanrong Bai