PE Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

RM4-5

Cat no : PE-65141

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse splenocytes using PE-65141

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2) and 0.25 ug PE Anti-Mouse CD4 (PE-65141, Clone:RM4-5) or 0.25 ug PE Rat IgG2a Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65141 targets CD4 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: BALB/c mouse thymocytes
• Full Name: CD4 antigen
• GenBank Accession Number: BC039137
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• RRID: AB_2883923
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
mouse	FC	J Thromb Haemost; Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1; Authors - Li-Ping Jiang
mouse	FC	Cell Death Discov; TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination; Authors - Qinmei Ma