PE Anti-Human CD163 (GHI/61)

CD163 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

GHI/61

Cat no : PE-65169

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

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Validation Data Gallery

• 

  FC experiment of human PBMCs using PE-65169

  1X10^6 human PBMCs were treated with FC receptor block then surface stained with CL405 Anti-Human CD14 and 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65169

  1X10^6 human PBMCs were treated with FC receptor block then surface stained with 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65169 targets CD163 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoprotein preparation from hairy cell leukemia spleen
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• RRID: AB_2883945
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Publications

Species	Application	Title
human	FC	Cell Death Discov; Suppression of the hyaluronic acid pathway induces M1 macrophages polarization via STAT1 in glioblastoma.; Authors - Tao Yan
	FC	Cell Mol Biol Lett; LINC00330/CCL2 axis-mediated ESCC TAM reprogramming affects tumor progression; Authors - Lijun Zhao