PE Mouse IgG2a Isotype Control (C1.18.4)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

C1.18.4

Cat no : PE-65208

Synonyms

IgG2a, IgG2a Isotype Control, PE Mouse IgG2a Isotype Control

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Validation Data Gallery

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  FC experiment of human PBMCs using PE-65208

  1X10^6 human PBMCs were surface stained with 0.2 ug PE Anti-Mouse IgG2a Isotype Control (PE-65208, Clone:C1.18.4) (red), or PE Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

PE-65208 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_2883959
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Publications

Species	Application	Title
	FC	Nat Microbiol; Multi-omics analyses of airway host-microbe interactions in chronic obstructive pulmonary disease identify potential therapeutic interventions; Authors - Zhengzheng Yan
	FC	Oncol Rep; MicroRNA‑8063 targets heterogeneous nuclear ribonucleoprotein AB to inhibit the self‑renewal of colorectal cancer stem cells via the Wnt/β‑catenin pathway.; Authors - Zheng-Quan Chen