PE Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

Publications

4

CloneNo.

3G3

Cat no : PE-65089

Synonyms

sf, scurfin, JM2, forkhead box P3, 3G3

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Validation Data Gallery

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  FC experiment of mouse splenocytes using PE-65089

  1X10^6 unstimulated and anti-CD3/CD28-stimulated (with 50 ng/ml TGF-β, 3 days) mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), and then intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (PE-65089, Clone:3G3).

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Tested Applications

• Positive FC (Intra) detected in: anti-CD3/CD28-stimulated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

PE-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: forkhead box P3
• GenBank Accession Number: BC132333
• Gene Symbol: Foxp3
• Gene ID (NCBI): 20371
• RRID: AB_2883883
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

Species	Application	Title
mouse	FC (Intra)	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC (Intra)	Hum Cell; Tetramerization of pyruvate kinase M2 attenuates graft-versus-host disease by inhibition of Th1 and Th17 differentiation; Authors - Meng Wang
		Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian
mouse	FC	J Adv Res; Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis; Authors - Yi Chen

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011