PE Anti-Mouse CD80 (B7-1) (16-10A1)
CD80 (B7-1) Monoclonal Antibody for FC
Host / Isotype
Armenian Hamster / IgG
Reactivity
mouse
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
16-10A1
Cat no : PE-65076
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse peritoneal macrophages |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 8 publications below |
Product Information
PE-65076 targets CD80 (B7-1) in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse |
Host / Isotype | Armenian Hamster / IgG |
Class | Monoclonal |
Type | Antibody |
Immunogen | CHO cell line transfected with mouse B7 (CD80) |
Full Name | CD80 antigen |
GenBank Accession Number | BC131959 |
Gene Symbol | Cd80 |
Gene ID (NCBI) | 12519 |
RRID | AB_2883872 |
Conjugate | PE Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)
Protocols
Product Specific Protocols | |
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FC protocol for PE CD80 (B7-1) antibody PE-65076 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Bioact Mater Turning foes to friends: Advanced "in situ nanovaccine" with dual immunoregulation for enhanced immunotherapy of metastatic triple-negative breast cancer | ||
Cell Rep Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells | ||
Nutrients The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype | ||
Acta Pharm Sin B Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy | ||
ACS Biomater Sci Eng Noninvasive Transdermal Administration of mRNA Vaccines Encoding Multivalent Neoantigens Effectively Inhibits Melanoma Growth | ||
J Adv Res Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis |