PE Anti-Mouse CD8a (53-6.7)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa


Mouse and More (1)




PE Fluorescent Dye



Cat no : PE-65069


BB154331, CD8 antigen, alpha chain, CD8a, CD8α, Ly 2, Ly 35, Ly B, Lyt 2

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 5 publications below

Product Information

PE-65069 targets CD8a in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Spleen cells or thymocyte membranes
Full Name CD8 antigen, alpha chain
GenBank Accession NumberBC030679
Gene Symbol Cd8a
Gene ID (NCBI) 12525
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).


Product Specific Protocols
FC protocol for PE CD8a antibody PE-65069Download protocol
Standard Protocols
Click here to view our Standard Protocols




Tailor-Made Autophagy Cascade Amplification Polymeric Nanoparticles for Enhanced Tumor Immunotherapy

Authors - Xuehua Long


Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Authors - Li Wu

J Mater Chem B

PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer

Authors - Lian Zhu

Asian J Pharm Sci

Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors

Authors - Xudong Fan

Cell Death Discov

TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination

Authors - Qinmei Ma