PE Anti-Human CD206 (15-2)

CD206 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

4

CloneNo.

15-2

Cat no : PE-65155

Synonyms

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Validation Data Gallery

• 

  FC experiment of human monocyte-derived dendritic cells using PE-65155

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul PE Anti-Human CD206 (PE-65155, Clone:15-2) (red) or PE-Mouse IgG1 isotype control (black). Cells were not fixed.

• 

  FC experiment of THP-1 using PE-65155

  THP-1 cells were differentiated into macrophages by incubation in the presence of phorbol 12-myristate 13-acetate (PMA). THP-1-derived macrophages were surface stained with PE Anti-Human CD206 (PE-65155, Clone: 15-2) (dashed line), or polarized in M2 macrophages by incubation with IL-4 (20 ng/ml) and IL-13 (20 ng/ml) for 48h and then surface stained with PE Anti-Human CD206 (PE-65155, Clone: 15-2) (red) or isotype control (blue, solid line). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human monocyte-derived dendritic cells cells, IL-4 and IL-13-stimulated THP-1-derived macrophages

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 4 publications below

Product Information

PE-65155 targets CD206 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified human mannose receptor
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: MRC1
• Gene ID (NCBI): 4360
• RRID: AB_2920521
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publications

Species	Application	Title
human	FC	Oncogene; Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells; Authors - Ying Zhan
	FC	J Agric Food Chem; Polysaccharide from Ziyang Selenium-Enriched Green Tea Prevents Obesity and Promotes Adipose Thermogenesis via Modulating the Gut Microbiota; Authors - Donglu Li
human	FC	Eur J Histochem; Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells in vitro via mediating macrophages M2 polarization; Authors - Xiangjun Lu
human	FC	Biomed Pharmacother; Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma; Authors - Tsung-Hua Hsieh