PE Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

3

CloneNo.

OKT3

Reviews (1)

Cat no : PE-65133

Synonyms

CD3, CD3E, T3E, TCRE

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Validation Data Gallery

• 

  FC experiment of human PBMCs using PE-65133

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65133

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone: OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

PE-65133 targets CD3 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human T cells
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3E
• Gene ID (NCBI): 916
• RRID: AB_2883916
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
	FC	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
human	FC	DNA Cell Biol; Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.; Authors - Yuan-Hao Wu
human	FC	Adv Sci (Weinh); Lipid Nanoparticle Delivery System for mRNA Encoding B7H3-redirected Bispecific Antibody Displays Potent Antitumor Effects on Malignant Tumors; Authors - Cheng Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Amelia (Verified Customer) (12-19-2022)

CD3 antibody worked as it should, good separation of positive/negative population.

• Applications: Flow Cytometry