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Recombinant Human CD33 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2138

Validation Data Gallery

  • Purity of Recombinant Human CD33 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD33 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CD33 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CD33 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD33 protein Asp18-His259 (Accession# AAH28152.1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 945
Accession AAH28152.1
PredictedSize 53.0 kDa
SDS-PAGE 60-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD33, also known as sialic acid-binding Ig-like lectin 3 (Siglec-3) or gp67, is a 67-kDa type I transmembrane glycoprotein of sialic acid-binding immunoglobulin-like lactic (Siglec) family, a discrete subset of the immunoglobulin (Ig) superfamily molecules. It contains an amino-terminal V-set Ig-like domain and a C2-set Ig-like domain in its extracellular region, a transmembrane region, and two conserved tyrosine-based inhibitory signaling motif in its cytoplasmic region. Alternative splicing of CD33 RNA leads to a shorter isoform. CD33 is expressed on myeloid progenitors, granulocytes, monocytes, macrophages, dendritic cells, mast cells, subsets of activated T- and NK cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

References:

1. E J Favaloro, et al. (1988) Br J Haematol. 69(2):163-71.
2. S D Freeman, et al. (1995) Blood. 85(8):2005-12.
3. C Vitale, et al. (1999) Proc Natl Acad Sci U S A. 96(26):15091-6. 
4. Trinidad Hernández-Caselles, et al. (2006) J Leukoc Biol. 79(1):46-58.
5. George S Laszlo, et al. (2014) Blood Rev. 28(4):143-53.