E-cadherin Polyclonal antibody

• Featured Product
• KD/KO Validated

E-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

2030

Reviews (4)

Cat no : 20874-1-AP

Synonyms

CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1

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Validation Data Gallery

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  WB analysis of A431 using 20874-1-AP

  WB result of E-cadherin antibody (20874-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.

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  WB analysis using 20874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20874-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20874-1-AP

  MCF-7 (E-cadherin+), A431 (E-cadherin+), HeLa (E-cadherin-), MDA-MB-231 (E-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 20874-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20874-1-AP (E-cadherin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20874-1-AP

  IP result of anti-E-cadherin (IP:20874-1-AP, 3ug; Detection:20874-1-AP 1:1000) with A431 cells lysate 3000ug.

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  IHC staining of mouse colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 20874-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 20874-1-AP (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 20874-1-AP

  FFPE adult mouse small intestine stained for E-cadherin (white, 20874-1-AP5), LYVE1 (green), and alpha-smooth muscle actin (red). E-cadherin marks intestinal epithelial cells, which regulate nutrient absorption. LYVE1 stains gut lymphatics, which absorb fat. Alpha-smooth muscle actin stains perivascular smooth muscle cells and the surrounding smooth muscle, which help propel food in the gut. Image credit: @Immunofluorescence on Instagram.

• 

  IF Staining of human breast cancer using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 20874-1-AP (E-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 20874-1-AP

  Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

• 

  IF Staining of mouse colon using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse colon using 20874-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 20874-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 20874-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using E-cadherin antibody (20874-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: mouse colon tissue, human breast cancer tissue, human colon tissue, human prostate cancer tissue, mouse skin tissue, rat colon tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse colon tissue, human breast cancer tissue, mouse small intestine tissue
• Positive IF-Fro detected in: mouse colon tissue
• Positive IF/ICC detected in: MCF-7 cells, A431 cells

Some bands between 80 and 120 kDa may be observed due to proteolytic cleavage.

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:200000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 1796 publications below
• IHC: See 328 publications below
• IF: See 377 publications below
• IP: See 1 publications below
• FC: See 3 publications below
• CoIP: See 7 publications below

Product Information

20874-1-AP targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E-cadherin fusion protein Ag14973
• Full Name: cadherin 1, type 1, E-cadherin (epithelial)
• Calculated Molecular Weight: 882 aa, 97 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC141838
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 999
• RRID: AB_10697811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Publications

Species	Application	Title
mouse	IF	Science; Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.; Authors - Yanpu Chen
human,mouse	WB,IHC	Mol Cancer; lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2; Authors - Jin Wang
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
human, rat, mouse	WB	Sci Transl Med; MG53-mediated cell membrane repair protects against acute kidney injury.; Authors - Pu Duann
mouse	WB	ACS Nano; Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.; Authors - Han Lin
human	WB	Nat Commun; UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis; Authors - Jeonghyun Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Davide (Verified Customer) (11-22-2022)

The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cholangiocyte organoids

E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)

FH

Sophie (Verified Customer) (02-07-2022)

Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000

FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

FH

Martin (Verified Customer) (12-13-2017)

• Applications: Western Blot, Immunofluorescence

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