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E-cadherin Polyclonal antibody

E-cadherin Polyclonal Antibody for WB, IP, IHC, IF-P, ELISA, IF-Fro, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF-P, CoIP, ELISA, IF-Fro, IF/ICC, Cell treatment

Conjugate

Unconjugated

Cat no : 20874-1-AP

Synonyms

CDH1, CD324, CAM 120/80, Cadherin-1, Cadherin 1



Tested Applications

Positive WB detected inA431 cells, DU 145 cells, mouse testis tissue, HCT 116 cells, MCF-7 cells, T-47D cells
Positive IP detected inA431 cells
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human colon tissue, mouse skin tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, human breast cancer tissue, mouse embryo tissue, mouse small intestine tissue
Positive IF-Fro detected inmouse colon tissue
Positive IF/ICC detected inMCF-7 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20874-1-AP targets E-cadherin in WB, IP, IHC, IF-P, CoIP, ELISA, IF-Fro, IF/ICC, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
RRIDAB_10697811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 20874-1-APDownload protocol
IHC protocol for E-cadherin antibody 20874-1-APDownload protocol
IF protocol for E-cadherin antibody 20874-1-APDownload protocol
IP protocol for E-cadherin antibody 20874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

Reversible reprogramming of cardiomyocytes to a fetal state drives heart regeneration in mice.

Authors - Yanpu Chen
human,mouseWB,IHC

Mol Cancer

lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2

Authors - Jin Wang
humanIF

Adv Mater

Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Authors - Zhuo Li
human, rat, mouseWB

Sci Transl Med

MG53-mediated cell membrane repair protects against acute kidney injury.

Authors - Pu Duann
mouseWB

ACS Nano

Biomimetic Nanomedicine-Triggered in Situ Vaccination for Innate and Adaptive Immunity Activations for Bacterial Osteomyelitis Treatment.

Authors - Han Lin
humanWB

Nat Commun

UBAP2 plays a role in bone homeostasis through the regulation of osteoblastogenesis and osteoclastogenesis

Authors - Jeonghyun Kim

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Davide (Verified Customer) (11-22-2022)

The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocyte organoids
E-cadherin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20874-1-AP)
FH

Sophie (Verified Customer) (02-07-2022)

Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
FH

Kishor (Verified Customer) (01-30-2019)

This antibody working very good for human cancer cells in western blotting (1:1000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Martin (Verified Customer) (12-13-2017)

  • Applications: Western Blot, Immunofluorescence