GAPDH Antibody 446 Publications

Mouse Monoclonal| Catalog number: 60004-1-Ig |CloneNo.: 1E6D9

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Size: 09µ/gram

Please visit your regions distributor:

Species specificity:
human, mouse, rat, zebrafish, yeast, C. elegans, cow, Cyprinus carpio, hamster, monkey, mussel P. viridis, pig, porcine, Spodoptera litura, Xenopus laevis

Positive WB detected in:
multi-cells/tissue, arabidopsis whole plant tissue, corn whole plant tissue, HEK-293 cells, HeLa cells, Raji cells, rice whole plant tissue, soybean whole plant tissue, wheat whole plant tissue, whole Nematode tissue, whole yeast cells, zebrafish tissue

Positive IP detected in:
HeLa Cells

Positive IHC detected in:
human kidney tissue, human breast cancer tissue

Positive IF detected in:
Ethacrynic acid treated HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:5000-1:20000
IP : 1:3000-1:20000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Purification method:
Protein A purification


PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
glyceraldehyde-3-phosphate dehydrogenase

Calculated molecular weight:
36 kDa

Observed molecular weight:
36 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

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