Caspase 3/p17/p19 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

2205

Reviews (8)

Cat no : 19677-1-AP

Synonyms

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

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Validation Data Gallery

• 

  WB analysis using 19677-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3/p17/p19 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19677-1-AP

  WB result of Caspase 3 antibody (19677-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells.

• 

  WB analysis of mouse spleen using 19677-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19677-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (CASP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 19677-1-AP

  IP result of anti-Caspase 3/p17/p19 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

• 

  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19677-1-AP (CASP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human teeth using 19677-1-AP

  Immunohistochemical analysis of paraffin-embedded human teeth tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse brain using 19677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 19677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Caspase 3/p17/p19 antibody (19677-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 19677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19677-1-AP (Caspase 3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 19677-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Retinal organoids using 19677-1-AP

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Vimentin with 60330-1-Ig at 1:500 dilution (green) and Caspase 3 using 19677-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse spleen tissue, HeLa cells, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue, mouse eye tissue
• Positive IF/ICC detected in: NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 2035 publications below
• IHC: See 201 publications below
• IF: See 101 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

19677-1-AP targets Caspase 3/p17/p19 in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, monkey, chicken, zebrafish, hamster, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa, 17 kDa, 19 kDa
• GenBank Accession Number: NM_004346
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• RRID: AB_10733244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
human	WB,IHC	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB	Nat Microbiol; Yersinia infection induces glucose depletion and AMPK-dependent inhibition of pyroptosis in mice; Authors - Yuanxin Yang
mouse	WB,IHC	Bioact Mater; Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection; Authors - Yumei Que
mouse,rat	WB	Nat Commun; Protective effects of Pt-N-C single-atom nanozymes against myocardial ischemia-reperfusion injury; Authors - Tianbao Ye
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (08-15-2024)

Done in milk. Many bands but the most intense one matches the p32 of the caspase 3

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

Caspase 3/p17/p19 Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:19677-1-AP)

FH

Alessandro (Verified Customer) (12-09-2023)

Reliable apoptosis Ab for IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC34

FH

Hala (Verified Customer) (04-12-2021)

works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepg2 treated with ethoposide

FH

Isha (Verified Customer) (02-03-2021)

Gave me crystal clear bands in kidney lysates. really happy with the product

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney

FH

Diane (Verified Customer) (02-02-2021)

Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cell lysates - glioblastoma primary

Caspase 3/p17/p19 Antibody Western Blot validation (1:500 dilution) in Cell lysates - glioblastoma primary (Cat no:19677-1-AP)

FH

Chao (Verified Customer) (03-12-2020)

Full-length but not cleaved isoform is detected by western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Kishor (Verified Customer) (01-30-2019)

It is an excellent antibody, worked every time when I used and got satisfactory results.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

Caspase 3/p17/p19 Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:19677-1-AP)

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