- Featured Product
- KD/KO Validated
Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (8)
WB, RIP, IP, IHC, IF, FC, ELISA
Cat no : 19677-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, mouse brain tissue, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 1638 publications below|
|IHC||See 162 publications below|
|IF||See 77 publications below|
|IP||See 1 publications below|
|ELISA||See 1 publications below|
|RIP||See 1 publications below|
19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Bovine, canine, chicken, duck, Goat, hamster, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32-35 kDa, 17 kDa, 19 kDa|
|GenBank accession number||NM_004346|
|Gene ID (NCBI)||836|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|FC protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sci Transl Med
PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.
UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.
Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms
Structural insight into the molecular mechanism of p53-mediated mitochondrial apoptosis.
Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.
Cell Death Differ
Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:1000.
Hala (Verified Customer) (04-12-2021)
works very well
Isha (Verified Customer) (02-03-2021)
Gave me crystal clear bands in kidney lysates. really happy with the product
Diane (Verified Customer) (02-02-2021)
Incubated overnight 4 degrees C. Secondary 1:2500. Excellent bands. Used Opti-4CN substrate kit for visualization.
Chao (Verified Customer) (03-12-2020)
Full-length but not cleaved isoform is detected by western blot
Kishor (Verified Customer) (01-30-2019)
It is an excellent antibody, worked every time when I used and got satisfactory results.