ARL13B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL13B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

800

Reviews (15)

Cat no : 17711-1-AP

Synonyms

ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B

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Validation Data Gallery

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  WB analysis using 17711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17711-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17711-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 17711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17711-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17711-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17711-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 17711-1-AP

  IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

• 

  IHC staining of mouse brain using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17711-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of NIH3T3 cells using 17711-1-AP

  IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

• 

  IF Staining of hTERT-RPE1 using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of MDCK using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of MDCK using 17711-1-AP

  Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG(H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 66200-1-Ig.

• 

  IF Staining of MDCK using 17711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 17711-1-AP

  IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

• 

  IF Staining of mouse embryonic fibroblasts using 17711-1-AP

  IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

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Tested Applications

• Positive WB detected in: L02 cells, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, rat liver tissue, rat kidney tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: mouse brain tissue, human kidney tissue, human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells, NIH3T3 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 85 publications below
• IHC: See 39 publications below
• IF: See 715 publications below
• IP: See 2 publications below
• FC: See 2 publications below

Product Information

17711-1-AP targets ARL13B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 40-48 kDa, 60 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• RRID: AB_2060867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Publications

Species	Application	Title
mouse	IF	Cell; The developmental basis of fingerprint pattern formation and variation; Authors - James D Glover
human	IF	Nat Biotechnol; Guided self-organization and cortical plate formation in human brain organoids.; Authors - Madeline A Lancaster
mouse	IF	Nature; Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.; Authors - David Willnow
mouse	IF	Science; Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.; Authors - Avishag Mytlis
human	IF	Nature; A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.; Authors - Adriana Sánchez-Danés
mouse	IF	Nature; Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.; Authors - Matthew B Johnson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ludovic (Verified Customer) (12-10-2023)

The perfect antibody, works each time!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500 to 1:1000
• Cell Tissue Type: Chodnrocytes

FH

Gagan (Verified Customer) (07-02-2023)

Antibody gives good staining for cilia.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mammalian cells - Hk-2

ARL13B Antibody Immunofluorescence validation (1:500 dilution) in Mammalian cells - Hk-2 (Cat no:17711-1-AP)

FH

Mi (Verified Customer) (05-29-2023)

An excellent antibody, it works pretty well in human white adipocytes!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: adipocytes

FH

Elisa (Verified Customer) (03-17-2023)

Great antibody for centrosome and cilia visualizationin RPE1 cells. Cells were fixed in cold methanol for 10' at -20C, rehydrated with PBS for 5', and permeabilized with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). Image show Arl13b (in magenta), DNA (Hoechst in blue) and g-tubulin (in green).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

ARL13B Antibody Immunofluorescence validation (1:400 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:17711-1-AP)

FH

Serhiy (Verified Customer) (12-21-2022)

Excellent for immunofluorescent staining of ciliary Arl13b in mouse cells. Image shows ciliary axonemes immunostained for Arl13b (red).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Fibroblasts, kidney collecting duct cells.

ARL13B Antibody Immunofluorescence validation (1:200 dilution) in Fibroblasts, kidney collecting duct cells. (Cat no:17711-1-AP)

FH

Kaviya (Verified Customer) (09-19-2022)

I use this antibody for IHC on brain tissue sections and it generally works well.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500

FH

Charlotte (Verified Customer) (07-29-2022)

NIH-3T3 stained in DAPI (nucleus), FITC (gamma tubulin), Cy3 (Arl13b). The last picture is the merged channels (FITC and Cy3). On the merged channel, we can conclude that all Cy3 signal is validated by the FITC one (gamma tubulin is present at the base of the primary cilia). Perfect antibody !

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

ARL13B Antibody Immunofluorescence validation (1/1000 dilution) in NIH-3T3 (Cat no:17711-1-AP)

FH

Sarah (Verified Customer) (02-16-2021)

Works beautifully in all cell types I've tried. Specifically great to see it work in primary cells (glioblastoma, pictured) as well as iPSCs

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Primary glioblastoma cells and iPSC

ARL13B Antibody Immunofluorescence validation (1:300 dilution) in Primary glioblastoma cells and iPSC (Cat no:17711-1-AP)

FH

deng (Verified Customer) (01-30-2020)

Very good for cilia staining

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1 : 250
• Cell Tissue Type: RPE cells

ARL13B Antibody Immunofluorescence, validation (1 : 250 dilution) in RPE cells (Cat no:17711-1-AP)

FH

Gabriela (Verified Customer) (01-01-2020)

Very specific antibody for primary cilia. We use it in chicken embryos spinal cord.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: spinal cord/Chicken

FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

This antibody works really well in human and mouse cell lines and in zebrafish samples, we used this antibody for WB, IF and Flow cytometry.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB and 1:200 for IF
• Cell Tissue Type: Brain Endothelial cells, brain pericytes, zebrafish embryos

ARL13B Antibody Western Blot,Immunofluorescence, validation (1:1000 for WB and 1:200 for IF dilution) in Brain Endothelial cells, brain pericytes, zebrafish embryos (Cat no:17711-1-AP)

FH

Rebeca (Verified Customer) (09-26-2019)

I have used this antibody for several immunofluorescence stainings of primary cilia. The antibody works beautifully, would definitely recommend!

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse pancreatic organoids

FH

Pavel (Verified Customer) (09-18-2019)

Strong ciliary staining in MDCK cells. Works both with PFA and MeOH fixation.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: MDCK cells

FH

Lindsey (Verified Customer) (02-04-2019)

Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells

ARL13B Antibody Immunofluorescence, validation (1:1000 dilution) in E11.25 Dorsal Root Ganglion/Embryonic Neural Crest Cells (Cat no:17711-1-AP)

FH

Kishor (Verified Customer) (01-30-2019)

I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 and 1:200
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

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