beta Actin Antibody 522 Publications

Mouse Monoclonal| Catalog number: 60008-1-Ig |CloneNo.: 7D2C10

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Freight/Packing

Size: 09µ/gram

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Species specificity:
Human, Mouse, Rat, Zebrafish, Pig, yeast, Baby Hamster Syrian, bovine, C. elegans, C. tubulosa, chicken, Drosophila, duck, fly, Rare minnows, rice

Positive WB detected in:
multi-cells/tissue, A549 cells, arabidopsis whole plant tissue, HEK-293 cells, HeLa cells, MCF7 cells, whole Nematode tissue, whole yeast cells

Positive IHC detected in:
human colon tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:2000-1:20000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Caprylic acid/ammonium sulfate precipitation

Isotype:
IgM

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
actin, beta

Calculated molecular weight:
375aa,42 kDa

Observed molecular weight:
42 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ACTB

Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin
Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. The isotype of this antibody is mouse IgM. It can be detected with the secondary antiody "SA00001-1" Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG(H+L).


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