P53 Polyclonal antibody

• Featured Product
• KD/KO Validated

P53 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

965

Reviews (9)

Cat no : 10442-1-AP

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

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Validation Data Gallery

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  WB analysis of SMMC-7721 using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10442-1-AP

  WB result of P53 antibody (10442-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

• 

  WB analysis using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10442-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10442-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 10442-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 10442-1-AP (P53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 10442-1-AP

  IP result of anti-P53 (IP:10442-1-AP, 4ug; Detection:10442-1-AP 1:1000) with A431 cells lysate 3000ug.

• 

  IHC staining of human ovary cancer using 10442-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10442-1-AP (P53 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 10442-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10442-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (10442-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, SMMC-7721 cells, MCF-7 cells, rat colon tissue, HEK-293 cells, HT-29 cells, HepG2 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 46 publications below
• WB: See 899 publications below
• IHC: See 85 publications below
• IF: See 82 publications below
• IP: See 25 publications below
• CoIP: See 21 publications below
• ChIP: See 10 publications below
• RIP: See 2 publications below

Product Information

10442-1-AP targets P53 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat, pig, rabbit, chicken, zebrafish, sheep, goat, artemia sinica
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P53 fusion protein Ag0698
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• RRID: AB_2206609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

Publications

Species	Application	Title
human	IHC	Nat Med; A biobank of patient-derived pediatric brain tumor models.; Authors - Sebastian Brabetz
rat,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB,IHC	Nat Commun; URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers; Authors - Zhiwen Ding
human	WB	Sci Transl Med; PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.; Authors - Tian-Yi Jiang
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	J Am Chem Soc; Acetylation Targeting Chimera Enables Acetylation of the Tumor Suppressor p53; Authors - Md Kabir

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laetitia (Verified Customer) (03-21-2024)

Finding antibodies specific to avian proteins is very difficult. I have tried different antibodies from different companies so far. This antibody is by far the best (cost efficient as well)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Epithelial chicken cells

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band on human lysates

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Kimberly (Verified Customer) (04-08-2022)

TRIS antigen retrieval

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: colon tissue, human and mouse

FH

Ryan (Verified Customer) (08-27-2021)

Worked well in HeLa cells and provided a nice clear band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3500
• Cell Tissue Type: HeLa Cells

FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band at the right size was detected. Good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

P53 Antibody Western Blot validation (1:500 dilution) in (Cat no:10442-1-AP)

FH

WEI (Verified Customer) (01-17-2020)

Weak band in heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Ben (Verified Customer) (09-26-2019)

It worked very well for our westerns.

• Applications: Western Blot,

FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western for human samples, moderate background/non specific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human embryonic kidney

FH

Josiah (Verified Customer) (02-27-2019)

Antibody performed superb in a Western Blot application. Used Licor IR secondary antibodies to label the p53 antibody along with ß-actin as a duplex assay and imaged on a Licor Odyssey FC.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T8

P53 Antibody Western Blot, validation (1:1000 dilution) in T8 (Cat no:10442-1-AP)

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