Validation Data Gallery
|Positive WB detected in||A431 cells, SMMC-7721 cells, MCF-7 cells, rat colon tissue, mouse spleen tissue, HEK-293 cells, mouse colon tissue, HT-29 cells|
|Positive IP detected in||A431 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10442-1-AP targets P53 in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, rat, Artemia sinica, chicken, goat, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||P53 fusion protein Ag0698|
|Full Name||tumor protein p53|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||BC003596|
|Gene ID (NCBI)||7157|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
1. What is p53?
P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.
2. FAQs and p53
a. I fail to detect p53 by western blotting
Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.
b. I fail to detect p53 in some cell lines by western blotting
Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.
c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size
p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.
Toxicol Appl Pharmacol
Int J Biochem Cell Biol
Sinoporphyrin sodium based sonodynamic therapy induces anti-tumor effects in hepatocellular carcinoma and activates p53/caspase 3 axis.
Biochem Cell Biol
Knockdown of RNF2 enhances radiosensitivity of lung squamous cell carcinoma cells.
Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.
Anterior Gradient-2 monoclonal antibody inhibits lung cancer growth and metastasis by upregulating p53 pathway and without exerting any toxicological effects: A preclinical study.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kimberly (Verified Customer) (04-08-2022)
TRIS antigen retrieval
Ryan (Verified Customer) (08-27-2021)
Worked well in HeLa cells and provided a nice clear band.
Fan (Verified Customer) (03-22-2021)
Mouse retinal tissue was used. Single band at the right size was detected. Good antibody.
WEI (Verified Customer) (01-17-2020)
Weak band in heart lysates
Ben (Verified Customer) (09-26-2019)
It worked very well for our westerns.
Sid (Verified Customer) (04-12-2019)
Product performed well in western for human samples, moderate background/non specific binding
Josiah (Verified Customer) (02-27-2019)
Antibody performed superb in a Western Blot application. Used Licor IR secondary antibodies to label the p53 antibody along with ß-actin as a duplex assay and imaged on a Licor Odyssey FC.