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  • KD/KO Validated

P53 Polyclonal antibody

P53 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse and More (5)





Cat no : 10442-1-AP


Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, mouse spleen tissue, SMMC-7721 cells, rat colon tissue, HEK-293 cells, mouse colon tissue, HepG2 cells, HT-29 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10442-1-AP targets P53 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, rat, Artemia sinica, chicken, goat, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P53 fusion protein Ag0698
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


Product Specific Protocols
WB protocol for P53 antibody 10442-1-APDownload protocol
IHC protocol for P53 antibody 10442-1-APDownload protocol
IF protocol for P53 antibody 10442-1-APDownload protocol
IP protocol for P53 antibody 10442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Med

A biobank of patient-derived pediatric brain tumor models.

Authors - Sebastian Brabetz

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng


p53 protects against alcoholic fatty liver disease via ALDH2 inhibition

Authors - Pengbo Yao

ACS Nano

PMID: 31670945

Authors - Ying Xu

Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Macarena Lucia (Verified Customer) (10-17-2022)

clear band on human lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000

Kimberly (Verified Customer) (04-08-2022)

TRIS antigen retrieval

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: colon tissue, human and mouse

Ryan (Verified Customer) (08-27-2021)

Worked well in HeLa cells and provided a nice clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3500
  • Cell Tissue Type: HeLa Cells

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band at the right size was detected. Good antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
P53 Antibody Western Blot validation (1:500 dilution) in (Cat no:10442-1-AP)

WEI (Verified Customer) (01-17-2020)

Weak band in heart lysates

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart

Ben (Verified Customer) (09-26-2019)

It worked very well for our westerns.

  • Applications: Western Blot,

Sid (Verified Customer) (04-12-2019)

Product performed well in western for human samples, moderate background/non specific binding

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human embryonic kidney

Josiah (Verified Customer) (02-27-2019)

Antibody performed superb in a Western Blot application. Used Licor IR secondary antibodies to label the p53 antibody along with ß-actin as a duplex assay and imaged on a Licor Odyssey FC.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T8
P53 Antibody Western Blot, validation (1:1000 dilution) in T8 (Cat no:10442-1-AP)