Lamin B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Lamin B1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

1104

Reviews (7)

Cat no : 12987-1-AP

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 12987-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12987-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12987-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12987-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12987-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12987-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 12987-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12987-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12987-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12987-1-AP

  IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug.

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  IHC staining of human liver cancer using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 12987-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human skin cancer using 12987-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 12987-1-AP

  Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of NIH/3T3 using 12987-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 12987-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, U-937 cells, mouse spleen tissue, K-562 cells, Y79 cells, mouse pancreas tissue, SH-SY5Y cells, mouse kidney tissue, mouse lung tissue, mouse eye tissue, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse heart tissue, human colon cancer tissue, human kidney tissue, human liver cancer tissue, human normal colon, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human skin cancer tissue
• Positive IF/ICC detected in: HepG2 cells, human skin cancer tissue, NIH/3T3 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 1035 publications below
• IHC: See 9 publications below
• IF: See 59 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• ChIP: See 2 publications below

Product Information

12987-1-AP targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, hamster, bombyx mori
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag3631
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• RRID: AB_2136290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Publications

Species	Application	Title
human	WB,IF	Nat Biotechnol; Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases; Authors - Matthew T N Yarnall
mouse	WB	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
human	IF	Nature; Chromothripsis drives the evolution of gene amplification in cancer.; Authors - Ofer Shoshani
	WB	Nature; Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1.; Authors - Yingfeng Li
human	WB	Mol Cancer; Diversifying the anthracycline class of anti-cancer drugs identifies aclarubicin for superior survival of acute myeloid leukemia patients; Authors - Xiaohang Qiao
mouse	WB	Immunity; 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages; Authors - Jun Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

Works great in human adipocytes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes

FH

Ning (Verified Customer) (01-28-2023)

Excellent antibody to probe lamin B1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Lamin B1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:12987-1-AP)

FH

Tsimafei (Verified Customer) (12-04-2022)

20µg of total protein were loaded. Incubation for 2h at room temperature. Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESCs

Lamin B1 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:12987-1-AP)

FH

Alejandro (Verified Customer) (08-07-2022)

Works fine for flow cytometry

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Mouse bone marrow derived macrophages

FH

Iram (Verified Customer) (09-04-2020)

Very sharp bands for nuclear protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

FH

Chun (Verified Customer) (12-05-2019)

Excellent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HEK293 and HaCaT cells

FH

Marco (Verified Customer) (09-02-2019)

good staining in conjunction with Alexa Fluor Plus 488 secondary

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human mesenchymal stem cells

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