TDP-43 Polyclonal antibody

• Featured Product
• KD/KO Validated

TDP-43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (6)

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM

Conjugate

Unconjugated

Publications

1521

Reviews (29)

Cat no : 10782-2-AP

Synonyms

TDP-43 (N-terminal), TDP 43, TARDBP, TDP43, TAR DNA-binding protein 43

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Validation Data Gallery

• 

  WB analysis using 10782-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10782-2-AP

  WB result of TDP-43 antibody (10782-2-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

• 

  IP experiment of HeLa using 10782-2-AP

  IP result of anti-TDP-43 (IP:10782-2-AP, 4ug; Detection:10782-2-AP 1:50000) with HeLa cells lysate 1520 ug.

• 

  IP experiment of HeLa cells using 10782-2-AP

  IP result of anti-TDP43 (10782-2-AP for IP and 60019-2-Ig for Detection).

• 

  IHC staining of human brain (FTLD-U) tissue using 10782-2-AP

  40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

• 

  IHC staining of human gliomas using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TDP-43 antibody (10782-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:584 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10782-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (10782-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:3000-1:12000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 39 publications below
• WB: See 700 publications below
• IHC: See 686 publications below
• IF: See 471 publications below
• IP: See 41 publications below
• ELISA: See 4 publications below
• CoIP: See 6 publications below
• ChIP: See 6 publications below
• RIP: See 5 publications below

Product Information

10782-2-AP targets TDP-43 in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, monkey, chicken, zebrafish, hamster, yeast, horse, seal
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_615042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Publications

Species	Application	Title
human	WB	Nature; Oxidative cyclization reagents reveal tryptophan cation-π interactions; Authors - Xiao Xie
mouse	IF	Cell; Mouse oocytes sequester aggregated proteins in degradative super-organelles; Authors - Gabriele Zaffagnini
human	WB	Lancet Neurol; A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.; Authors - Ilse Gijselinck
mouse	IF	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
mouse	IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
human	WB	Nature; MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.; Authors - Roberto Simone

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Scott (Verified Customer) (10-22-2024)

10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size but with two bands, blue bars, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: TDP43 Q331K Mouse Brain Lysate

TDP-43 Antibody Western Blot validation (1:2000 dilution) in TDP43 Q331K Mouse Brain Lysate (Cat no:10782-2-AP)

FH

Makenna (Verified Customer) (07-16-2024)

Didn't work well for western blot, had high background

• Applications: Western Blot

FH

Paloma (Verified Customer) (03-26-2024)

We love this item since we start to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000-1:8000
• Cell Tissue Type: IHQ for neuroscience

FH

MANOHAR (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Paloma (Verified Customer) (02-27-2024)

We have been using the TDP43 antibody for diagnosis of neurodegenerative diseases for a long time. We like this proteintech antibody very much, because its format and dilution makes our work much easier. In addition, the image it gives us after IHC is beautiful and helps us in the diagnosis.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000-1:8000
• Cell Tissue Type: IHQ for neuroscience

FH

David (Verified Customer) (01-02-2024)

Top notch antibody, single band at predicted molecular weight. Gold standard.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y lysate

FH

Parijat (Verified Customer) (09-09-2023)

Good antibody for IF

• Applications: Immunofluorescence

TDP-43 Antibody Immunofluorescence validation ( dilution) in (Cat no:10782-2-AP)

FH

Florencia (Verified Customer) (07-17-2023)

This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: iPSC-derived neurons

FH

Pauline (Verified Customer) (02-23-2022)

everything ok

• Applications: Immunofluorescence
• Cell Tissue Type: HeLa

FH

Xin (Verified Customer) (01-23-2022)

Very good in WB with a band of around 45 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

FH

Jessica (Verified Customer) (06-10-2021)

good for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000

FH

Ana (Verified Customer) (03-14-2021)

TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary human fibrosblasts

TDP-43 Antibody Western Blot validation (1:1000 dilution) in Primary human fibrosblasts (Cat no:10782-2-AP)

FH

Yasuyo (Verified Customer) (01-20-2021)

Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: fibroblast cell

FH

MEIMEI (Verified Customer) (11-12-2020)

the antibody work prefect for my staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: iPSCs

FH

Stephane (Verified Customer) (10-31-2020)

Good product for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Neuron

TDP-43 Antibody Immunofluorescence validation (1:100 dilution) in Neuron (Cat no:10782-2-AP)

FH

Marina (Verified Customer) (10-25-2020)

TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

TDP-43 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:10782-2-AP)

FH

Uxoa (Verified Customer) (02-18-2020)

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

TDP-43 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:10782-2-AP)

FH

Paul (Verified Customer) (01-15-2020)

Produces good IF images with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

FH

Laura (Verified Customer) (01-10-2020)

Works very well

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300

FH

Benjamin (Verified Customer) (01-07-2020)

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2500, 1:100
• Cell Tissue Type: HEK293T

FH

Apoorva (Verified Customer) (12-18-2019)

It has great specificity over wide range of species.

• Applications: Western Blot,

FH

Tilly (Verified Customer) (11-27-2019)

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
• Cell Tissue Type: Mouse brain tissue

FH

Azita (Verified Customer) (10-04-2019)

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
• Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)

FH

Francisco (Verified Customer) (09-28-2019)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse

FH

Alinda (Verified Customer) (09-11-2019)

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SHSY5Y

TDP-43 Antibody Western Blot, validation (1:1000 dilution) in SHSY5Y (Cat no:10782-2-AP)

FH

David (Verified Customer) (07-18-2019)

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
• Cell Tissue Type: Neuroblastoma, iPSC-derived neurons

FH

George (Verified Customer) (07-07-2019)

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Spinal Cord/Brain slices

FH

Elena (Verified Customer) (08-17-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK-293 cells

FH

Petra (Verified Customer) (03-06-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

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