TDP-43 Polyclonal antibody

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  WB analysis using 10782-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10782-2-AP (TDP-43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10782-2-AP

  WB result of TDP-43 antibody (10782-2-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

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  IP experiment of HeLa using 10782-2-AP

  IP result of anti-TDP-43 (IP:10782-2-AP, 4ug; Detection:10782-2-AP 1:50000) with HeLa cells lysate 1520 ug.

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  IP experiment of HeLa cells using 10782-2-AP

  IP result of anti-TDP43 (10782-2-AP for IP and 60019-2-Ig for Detection).

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  IHC staining of human brain (FTLD-U) tissue using 10782-2-AP

  40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

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  IHC staining of human gliomas using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 (N-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10782-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10782-2-AP (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TDP-43 antibody (10782-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:6000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 10782-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (10782-2-AP) at dilution of 1:584 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10782-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (10782-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of HeLa using 10782-2-AP

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of TDP-43 Antibody(10782-2-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

• Featured Product
• KD/KO Validated

TDP-43 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA

Cat No. 10782-2-AP

Publications(1552)

Reviews (31)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (6)

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM

Synonyms

TDP-43 (N-terminal), TDP 43, TARDBP, TDP43, TAR DNA-binding protein 43

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, C2C12 cells, Neuro-2a cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human brain (FTLD-U) tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells
• Positive ChIP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:3000-1:12000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Chromatin immunoprecipitation (ChIP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 40 publications below
• WB: See 716 publications below
• IHC: See 692 publications below
• IF: See 485 publications below
• IP: See 41 publications below
• ELISA: See 4 publications below
• CoIP: See 7 publications below
• ChIP: See 7 publications below
• RIP: See 5 publications below

Product Information

10782-2-AP targets TDP-43 in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat, monkey, chicken, zebrafish, hamster, yeast, horse, seal
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• RRID: AB_615042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13148
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Publications

Species	Application	Title
human	WB	Nature; Oxidative cyclization reagents reveal tryptophan cation-π interactions; Authors - Xiao Xie
mouse	IF	Cell; Mouse oocytes sequester aggregated proteins in degradative super-organelles; Authors - Gabriele Zaffagnini
human	WB	Lancet Neurol; A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.; Authors - Ilse Gijselinck
mouse	IF	Cell Res; Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies; Authors - Liang Guo
mouse	IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
human	IF	Nature; TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.; Authors - X Rosa Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paloma (Verified Customer) (03-13-2025)

We love the results of this antibody

• Applications: Immunohistochemistry
• Primary Antibody Dilution: TDP43
• Cell Tissue Type: Brain

FH

Jose (Verified Customer) (02-24-2025)

N/A

• Applications: Cell culture

FH

Kevin (Verified Customer) (01-31-2025)

Worked well for Opal staining of TDP-43 in human brain tissue sections and visualization on confocal

• Applications: Immunohistochemistry
• Cell Tissue Type: Brain section

FH

Scott (Verified Customer) (10-22-2024)

10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size but with two bands, blue bars, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: TDP43 Q331K Mouse Brain Lysate

TDP-43 Antibody Western Blot validation (1:2000 dilution) in TDP43 Q331K Mouse Brain Lysate (Cat no:10782-2-AP)

FH

Makenna (Verified Customer) (07-16-2024)

Didn't work well for western blot, had high background

• Applications: Western Blot

FH

Paloma (Verified Customer) (03-26-2024)

We love this item since we start to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000-1:8000
• Cell Tissue Type: IHQ for neuroscience

FH

MANOHAR (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

David (Verified Customer) (01-02-2024)

Top notch antibody, single band at predicted molecular weight. Gold standard.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y lysate

FH

Parijat (Verified Customer) (09-09-2023)

Good antibody for IF

• Applications: Immunofluorescence

TDP-43 Antibody Immunofluorescence validation ( dilution) in (Cat no:10782-2-AP)

FH

Florencia (Verified Customer) (07-17-2023)

This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: iPSC-derived neurons

FH

Pauline (Verified Customer) (02-23-2022)

everything ok

• Applications: Immunofluorescence
• Cell Tissue Type: HeLa

FH

Xin (Verified Customer) (01-23-2022)

Very good in WB with a band of around 45 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

FH

Jessica (Verified Customer) (06-10-2021)

good for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000

FH

Ana (Verified Customer) (03-14-2021)

TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary human fibrosblasts

TDP-43 Antibody Western Blot validation (1:1000 dilution) in Primary human fibrosblasts (Cat no:10782-2-AP)

FH

Yasuyo (Verified Customer) (01-20-2021)

Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: fibroblast cell

FH

MEIMEI (Verified Customer) (11-12-2020)

the antibody work prefect for my staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: iPSCs

FH

Stephane (Verified Customer) (10-31-2020)

Good product for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Neuron

TDP-43 Antibody Immunofluorescence validation (1:100 dilution) in Neuron (Cat no:10782-2-AP)

FH

Marina (Verified Customer) (10-25-2020)

TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

TDP-43 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:10782-2-AP)

FH

Uxoa (Verified Customer) (02-18-2020)

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

TDP-43 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:10782-2-AP)

FH

Paul (Verified Customer) (01-15-2020)

Produces good IF images with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

FH

Laura (Verified Customer) (01-10-2020)

Works very well

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300

FH

Benjamin (Verified Customer) (01-07-2020)

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2500, 1:100
• Cell Tissue Type: HEK293T

FH

Apoorva (Verified Customer) (12-18-2019)

It has great specificity over wide range of species.

• Applications: Western Blot,

FH

Tilly (Verified Customer) (11-27-2019)

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
• Cell Tissue Type: Mouse brain tissue

FH

Azita (Verified Customer) (10-04-2019)

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
• Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)

FH

Francisco (Verified Customer) (09-28-2019)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse

FH

Alinda (Verified Customer) (09-11-2019)

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SHSY5Y

TDP-43 Antibody Western Blot, validation (1:1000 dilution) in SHSY5Y (Cat no:10782-2-AP)

FH

David (Verified Customer) (07-18-2019)

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
• Cell Tissue Type: Neuroblastoma, iPSC-derived neurons

FH

George (Verified Customer) (07-07-2019)

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Spinal Cord/Brain slices

FH

Elena (Verified Customer) (08-17-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK-293 cells

FH

Petra (Verified Customer) (03-06-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue