IFT88 Antibody

KD/KO Validated

Antibody Catalog number Type Applications
IFT88 13967-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB

Among its many functions, IFT88 helps mediate intraflagellar transport, which is essential for the assembly and maintenance of cilia and flagella in many organisms. The Proteintech polyclonal IFT88 antibody has appeared in over 190 publications to date.

One of these is related to Kif3 protein, and its role in mammalian Hedgehog (Hh) signaling, where researchers used anti‑IFT88 antibody as a marker for cilium retraction along with acetylated tubulin in IF staining.¹

IFT88 antibody validated in Immunofluorescent

Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at a dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG (H+L) for 66200-1-Ig

IFT88 antibody validated in western blot

HEK-293 cells were subjected to SDS PAGE followed by western blot with IFT88 antibody (13967-1-AP) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Loading control antibodies

 

GAPDH Antibody mouse monoclonal GAPDH antibody WB analysis of HeLa cells
Catalog no.: 60004-1-Ig

GAPDH is commonly used as a protein loading control in western blot due to its consistently high expression in most cell types. This enzyme participates in several cellular events such as glycolysis, DNA repair, and apoptosis.

Proteintech monoclonal GAPDH antibodies are raised against a whole-protein antigen of human origin and have over 4,960 citations.

Beta Actin Antibody (KD/KO validated) WB analysis of Jurkat cells using using beta actin antibody (66009-1-Ig)
Catalog no.: 66009-1-Ig 

Beta-actin is usually used as a loading control due to its broad and consistent expression across all eukaryotic cell types and the fact that expression levels of this protein are not affected by most experimental treatments.

66009-1-Ig has been cited in over 2,460 publications and has wide species reactivity.

 


 

References

1. Primary cilia regulate mTORC1 activity and cell size through Lkb1, (PMID: 20972424).