acetylated Tubulin(Lys40) Antibody 4 Publications

Mouse Monoclonal| Catalog number: 66200-1-Ig |CloneNo.: 7E5H8

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Freight/Packing

Con: 105 μg/150 μl

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Species specificity:
human, mouse, rat, dog, pig

Positive WB detected in:
L02 cells

Positive IHC detected in:
human lung cancer tissue, human gliomas tissue, human lung cancer tissue

Positive IF detected in:
MDCK cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
tubulin, alpha 1a

Calculated molecular weight:
52 kDa

Observed molecular weight:
55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
TUBA1A

Synonyms
acetylated alpha tubulin, Acetylated tubulin, Acetylated tubulin (Lys40), acetylated-a-tubulin, acetyl-a-tubulin, alpha tubulin, Alpha tubulin 3, tubulin
Background

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549)


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