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IFT88 Polyclonal antibody
IFT88 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 13967-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells|
|Positive IP detected in||knockout cells and WT cells, HEK-293 cells|
|Positive IHC detected in||human heart tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, hTERT-RPE1 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13967-1-AP targets IFT88 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, rat, canine, chicken, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT88 fusion protein Ag4980|
|Full Name||intraflagellar transport 88 homolog (Chlamydomonas)|
|Calculated molecular weight||94 kDa|
|Observed molecular weight||88-95 kDa|
|GenBank accession number||BC030776|
|Gene ID (NCBI)||8100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.
|Product Specific Protocols|
|WB protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IHC protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IF protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IP protocol for IFT88 antibody 13967-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Failure to reabsorb the primary cilium induces cellular senescence.
Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.
Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.
Am J Hum Genet
Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and a Range of Ciliopathy Phenotypes in Humans.
661W Photoreceptor Cell Line as a Cell Model for Studying Retinal Ciliopathies.
Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Stephen (Verified Customer) (02-08-2022)
works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin
Boyan (Verified Customer) (01-31-2019)
Excellent. Works quite well both in WB and IF.