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IFT88 Polyclonal antibody
IFT88 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13967-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells|
|Positive IP detected in||knockout cells and WT cells, HEK-293 cells|
|Positive IHC detected in||human heart tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, hTERT-RPE1 cells, C2C12 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13967-1-AP targets IFT88 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, rat, chicken, zebrafish, pig, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT88 fusion protein Ag4980|
|Full Name||intraflagellar transport 88 homolog (Chlamydomonas)|
|Calculated molecular weight||94 kDa|
|Observed molecular weight||88-95 kDa|
|GenBank accession number||BC030776|
|Gene ID (NCBI)||8100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.
|Product Specific Protocols|
|WB protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IHC protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IF protocol for IFT88 antibody 13967-1-AP||Download protocol|
|IP protocol for IFT88 antibody 13967-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.
Functional interaction between autophagy and ciliogenesis.
Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.
A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Complex interactions between genes controlling trafficking in primary cilia.
Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Serhiy (Verified Customer) (01-04-2023)
This antibody detects Ift88 in ciliary axonemes of immunofluorescently stained IMCD3 cells (atteched image: red Ift88; green - gamma-tubulin) and mouse fibroblasts. We also tried to detect endogenous Ift88 in NIH3T3 cells by western blots and found that antibody detects several non-specific bands in addition to apparently specific one between the 75-100 kDa markers.
Charlotte (Verified Customer) (07-26-2022)
We have revealed IFT88 after stripping a couple of times. The signal remains nice and the unspecific bands might be due to lower stripping efficiency
Stephen (Verified Customer) (02-08-2022)
works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin
Boyan (Verified Customer) (01-31-2019)
Excellent. Works quite well both in WB and IF.