IFT88 Polyclonal antibody

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  WB analysis using 13967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of knockout cells and WT cells using 13967-1-AP

  IP and WB result of IFT88 (13967-1-AP) from Dr. Corbit, Kevin. Knockout cells and WT cells.

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  IP experiment of HEK-293 using 13967-1-AP

  IP result of anti-IFT88 (IP:13967-1-AP, 3ug; Detection:13967-1-AP 1:800) with HEK-293 cells lysate 1500ug.

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  IHC staining of rat heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13967-1-AP (IFT88 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13967-1-AP (IFT88 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of hTERT-RPE1 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT88 antibody (13967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of C2C12 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT88 antibody (13967-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

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  IF Staining of hTERT-RPE1 using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated Goat anti-Mouse IgG(H+L) for 66200-1-Ig.

• Featured Product
• KD/KO Validated

The IFT88 polyclonal antibody (13967-1-AP) is the highest cited IFT88 antibody in the market with 380 citations.

Cat No. 13967-1-AP

Publications(380)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cell, MDCK cells, mouse thymus tissue
• Positive IP detected in: knockout cells and WT cells, HEK-293 cells
• Positive IHC detected in: mouse heart tissue, human pancreas tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells, MDCK cells, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 51 publications below
• WB: See 156 publications below
• IHC: See 8 publications below
• IF: See 257 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

13967-1-AP targets IFT88 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT88 fusion protein Ag4980
• Full Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC030776
• Gene Symbol: IFT88
• Gene ID (NCBI): 8100
• RRID: AB_2121979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13099
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Publications

Species	Application	Title
mouse	IF	Nature; Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.; Authors - Firestone Ari J AJ
mouse	WB	Nature; Functional interaction between autophagy and ciliogenesis.; Authors - Pampliega Olatz O
chicken	IF	Science; Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.; Authors - Raman M Das
human	IF	Nat Genet; Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.; Authors - Carol-Anne Martin
mouse	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Nat Genet; Complex interactions between genes controlling trafficking in primary cilia.; Authors - Ocbina Polloneal Jymmiel R PJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (04-08-2024)

Good Antibody for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

FH

Serhiy (Verified Customer) (01-04-2023)

This antibody detects Ift88 in ciliary axonemes of immunofluorescently stained IMCD3 cells (atteched image: red Ift88; green - gamma-tubulin) and mouse fibroblasts. We also tried to detect endogenous Ift88 in NIH3T3 cells by western blots and found that antibody detects several non-specific bands in addition to apparently specific one between the 75-100 kDa markers.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100 (IF), 1:1000 (WB)
• Cell Tissue Type: NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells

IFT88 Antibody Immunofluorescence validation (1:100 (IF), 1:1000 (WB) dilution) in NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells (Cat no:13967-1-AP)

FH

Charlotte (Verified Customer) (07-26-2022)

We have revealed IFT88 after stripping a couple of times. The signal remains nice and the unspecific bands might be due to lower stripping efficiency

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

IFT88 Antibody Western Blot,Immunofluorescence validation (1/500 dilution) in NIH-3T3 (Cat no:13967-1-AP)

FH

Stephen (Verified Customer) (02-08-2022)

works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human RPE-1 cells

IFT88 Antibody Immunofluorescence validation (1:500 dilution) in Human RPE-1 cells (Cat no:13967-1-AP)

FH

Boyan (Verified Customer) (01-31-2019)

Excellent. Works quite well both in WB and IF.

• Applications: Western Blot, Immunofluorescence,