IFT88 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT88 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (4)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

266

Reviews (2)

Cat no : 13967-1-AP

Synonyms

IFT88, TPR repeat protein 10, TTC10

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Validation Data Gallery

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  WB analysis using 13967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of knockout cells and WT cells using 13967-1-AP

  IP and WB result of IFT88 (13967-1-AP) from Dr. Corbit, Kevin. Knockout cells and WT cells.

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  IP experiment of HEK-293 using 13967-1-AP

  IP Result of anti-IFT88 (IP:13967-1-AP, 3ug; Detection:13967-1-AP 1:800) with HEK-293 cells lysate 1500ug.

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  IHC staining of human heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13967-1-AP (IFT88 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human pancreas using 13967-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13967-1-AP (IFT88 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 cells using 13967-1-AP

  IF result of anti-IFT88 (13967-1-AP) from Dr. Corbit, Kevin and Dr. Andrew Kodani.

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  IF Staining of MDCK using 13967-1-AP

  Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated AffiniPure Goat anti-Mouse IgG(H+L) for 66200-1-Ig.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, MDCK cells, NIH/3T3 cells
• Positive IP detected in: knockout cells and WT cells, HEK-293 cells
• Positive IHC detected in: human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells, hTERT-RPE1 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 38 publications below
• WB: See 100 publications below
• IHC: See 5 publications below
• IF: See 190 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

13967-1-AP targets IFT88 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse, rat, canine, chicken, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT88 fusion protein Ag4980
• Full Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Calculated molecular weight: 94 kDa
• Observed molecular weight: 88-95 kDa
• GenBank accession number: BC030776
• Gene symbol: IFT88
• Gene ID (NCBI): 8100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Publications

Species	Application	Title
human	WB	FASEB J; Failure to reabsorb the primary cilium induces cellular senescence.; Authors - Elizabeth P Jeffries ; KD Validated
mouse	IF	PLoS Genet; Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.; Authors - Emily Bowie
mouse	IF	Dev Cell; Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation.; Authors - Chia-Hsiang Chang ; KD Validated
human	IF	Am J Hum Genet; Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and a Range of Ciliopathy Phenotypes in Humans.; Authors - Ranad Shaheen
mouse	WB,IF	Front Genet; 661W Photoreceptor Cell Line as a Cell Model for Studying Retinal Ciliopathies.; Authors - Gabrielle Wheway
human	IF	Ann Neurol; Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis.; Authors - Hyowon Hong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Stephen (Verified Customer) (02-08-2022)

works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human RPE-1 cells

IFT88 Antibody Immunofluorescence validation (1:500 dilution) in Human RPE-1 cells (Cat no:13967-1-AP)

FH

Boyan (Verified Customer) (01-31-2019)

Excellent. Works quite well both in WB and IF.

• Applications: Western Blot, Immunofluorescence,

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