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  • KD/KO Validated

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, Canine, rat and More (2)





Cat no : 13615-1-AP


hIFT20, IFT20

Tested Applications

Positive WB detected inHEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

Western Blot (WB)WB : 1:300-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13615-1-AP targets IFT20 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, Canine, rat samples.

Tested Reactivity human, mouse, Canine, rat
Cited Reactivityhuman, mouse, rat, zebrafish, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT20 fusion protein Ag4521
Full Name intraflagellar transport 20 homolog (Chlamydomonas)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-18 kDa
GenBank Accession NumberBC038094
Gene Symbol IFT20
Gene ID (NCBI) 90410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.


Product Specific Protocols
WB protocol for IFT20 antibody 13615-1-APDownload protocol
IHC protocol for IFT20 antibody 13615-1-APDownload protocol
IF protocol for IFT20 antibody 13615-1-APDownload protocol
IP protocol for IFT20 antibody 13615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu

Sci Adv

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Authors - Molly M Wilson

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu

Proc Natl Acad Sci U S A

EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.

Authors - Vincent J Guen
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and IF for cilia (1:200) and got good results.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells

Boyan (Verified Customer) (03-15-2019)

Excellent for IF labelling of the Golgi under PFA fixation.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: MEF