• Featured Product
  • KD/KO Validated

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, Canine, rat and More (2)





Cat no : 13615-1-AP


hIFT20, IFT20

Tested Applications

Positive WB detected inHEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

Western Blot (WB)WB : 1:300-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13615-1-AP targets IFT20 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, Canine, rat samples.

Tested Reactivity human, mouse, Canine, rat
Cited Reactivityhuman, mouse, rat, canine, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT20 fusion protein Ag4521
Full Name intraflagellar transport 20 homolog (Chlamydomonas)
Calculated molecular weight 15 kDa
Observed molecular weight 15-18 kDa
GenBank accession numberBC038094
Gene symbol IFT20
Gene ID (NCBI) 90410
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.


Product Specific Protocols
WB protocol for IFT20 antibody 13615-1-APDownload protocol
IHC protocol for IFT20 antibody 13615-1-APDownload protocol
IF protocol for IFT20 antibody 13615-1-APDownload protocol
IP protocol for IFT20 antibody 13615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



JCI Insight

Hypomorphic mutations of TRIP11 cause odontochondrodysplasia.

Authors - Anika Wehrle

Cell Death Differ

The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases.

Authors - Francesca Finetti
  • KD Validated

BMC Cancer

Associations of IFT20 and GM130 protein expressions with clinicopathological features and survival of patients with lung adenocarcinoma.

Authors - Lianfeng Li

Cell Rep

The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.

Authors - Asma Boukhalfa

Cell Rep

Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis.

Authors - Marina Peralta
  • KO Validated

Cell Stress

The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow.

Authors - Naïma Zemirli


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and IF for cilia (1:200) and got good results.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells

Boyan (Verified Customer) (03-15-2019)

Excellent for IF labelling of the Golgi under PFA fixation.

  • Applications: Immunofluorescence,
  • Cell Tissue Type: MEF