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IFT20 Polyclonal antibody
IFT20 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 13615-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human endometrial cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:300-1:800|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13615-1-AP targets IFT20 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, Canine, rat samples.
|Tested Reactivity||human, mouse, Canine, rat|
|Cited Reactivity||human, mouse, rat, canine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT20 fusion protein Ag4521|
|Full Name||intraflagellar transport 20 homolog (Chlamydomonas)|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15-18 kDa|
|GenBank accession number||BC038094|
|Gene ID (NCBI)||90410|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.
|Product Specific Protocols|
|WB protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IHC protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IF protocol for IFT20 antibody 13615-1-AP||Download protocol|
|IP protocol for IFT20 antibody 13615-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hypomorphic mutations of TRIP11 cause odontochondrodysplasia.
Cell Death Differ
The intraflagellar transport protein IFT20 controls lysosome biogenesis by regulating the post-Golgi transport of acid hydrolases.
Associations of IFT20 and GM130 protein expressions with clinicopathological features and survival of patients with lung adenocarcinoma.
The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium.
Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis.
The primary cilium protein folliculin is part of the autophagy signaling pathway to regulate epithelial cell size in response to fluid flow.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
kes (Verified Customer) (01-17-2022)
I used it for WB(1:1000) and IF for cilia (1:200) and got good results.
Boyan (Verified Customer) (03-15-2019)
Excellent for IF labelling of the Golgi under PFA fixation.