Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19304-1-AP targets IFT122 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| Tested Reactivity | human, mouse, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6860 Product name: Recombinant human IFT122 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-359 aa of BC004238 Sequence: MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGLWSPEQKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREERNDILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELVKKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSGVKEREWQME Predict reactive species |
| Full Name | intraflagellar transport 122 homolog (Chlamydomonas) |
| Calculated Molecular Weight | 142 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC004238 |
| Gene Symbol | IFT122 |
| Gene ID (NCBI) | 55764 |
| RRID | AB_2714184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBG6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IFT122 antibody 19304-1-AP | Download protocol |
| WB protocol for IFT122 antibody 19304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IFT122 Polyclonal antibody (Cat# 19304-1-AP) has 3 citations published in Cell, Human Molecular Genetics, and eLife. The associated research fields include cilia biology, ciliary protein trafficking, ciliopathy, and cell cycle regulation through primary cilia excision.
| Species | Application | Title |
|---|---|---|
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Hum Mol Genet Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. | ||
Elife A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia. |
Reviews
What customers say
The sole review for 19304-1-AP is a 5-star rating from a researcher who used it at 1:200 dilution on primary myoblasts for immunofluorescence. They described it as a high-quality antibody that works well for both immunofluorescence and expansion microscopy, praising its very low background and high specificity. Overall, the feedback is strongly positive, highlighting reliable performance and clean signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lola (Verified Customer) (10-23-2025) | High quality antibody. Works well for immunofluorescence and expansion microscopy. Very low background and high specificity.
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