IFT88 Monoclonal antibody

• Featured Product
• KD/KO Validated

IFT88 Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

4A4G5

Reviews (1)

Cat no : 60227-1-Ig

Synonyms

IFT88, TPR repeat protein 10, TTC10

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Validation Data Gallery

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  WB analysis of human testis using 60227-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60227-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 60227-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 60227-1-Ig

  IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug.

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  IHC staining of human kidney using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 60227-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Human testis, tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human kidney tissue, human heart tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

The immunogen of 60227-1-Ig is IFT88 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFT88 fusion protein Ag4980
• Full Name: intraflagellar transport 88 homolog (Chlamydomonas)
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 88-95 kDa
• GenBank Accession Number: BC030776
• Gene Symbol: IFT88
• Gene ID (NCBI): 8100
• RRID: AB_11042449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; Crosstalk between the COX2-PGE2-EP4 signaling pathway and primary cilia in osteoblasts after mechanical stimulation.; Authors - Yan-Hui Li ; KD Validated
mouse	IF	Cell Cycle; Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells.; Authors - Saul S Siller
human	WB	Front Cell Dev Biol; Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.; Authors - Jiawei Li
mouse	WB	Front Mol Biosci; Primary cilia-mediated regulation of microglial secretion in Alzheimer's disease; Authors - Seungun Yeo ; KD Validated
mouse	WB	J Cell Physiol; Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.; Authors - Caleb S Hayes
human	WB	Molecules; 2-IPMA Ameliorates PM2.5-Induced Inflammation by Promoting Primary Ciliogenesis in RPE Cells; Authors - Ji Yeon Choi ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

k (Verified Customer) (10-26-2023)

I used it with TBST+5% BSA over night for WB analysis.

• Applications: Western Blot
• Primary Antibody Dilution: 1;500
• Cell Tissue Type: HePG2, Hela

IFT88 Antibody Western Blot validation (1;500 dilution) in HePG2, Hela (Cat no:60227-1-Ig)