IFT27 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15017-1-AP

Synonyms

RABL4, Rab-like protein 4, Rab like protein 4, Putative GTP-binding protein RAY-like, BBS19

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Validation Data Gallery

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  WB analysis of human RPE cells using 15017-1-AP

  WB result of anti-RABL4 (15017-1-AP) with human RPE cells (siRNA and control) by Dr. Gerald Liew, Maxence Nachury's lab.

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  IHC staining of human brain using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15017-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15017-1-AP (IFT27 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 15017-1-AP

  IF result (cytoplasm and the spindle midzone stain) of anti-RABL4 (IFT27, 15017-1-AP, 1:50) with telophase hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: human RPE cells
• Positive IHC detected in: human brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

15017-1-AP targets IFT27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT27 fusion protein Ag6967
• Full Name: RAB, member of RAS oncogene family-like 4
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC000566
• Gene Symbol: RABL4/IFT27
• Gene ID (NCBI): 11020
• RRID: AB_2878100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman ; KD Validated
human	WB	Mol Cell Proteomics; CRISPR/Cas9-mediated genomic editing of Cluap1/IFT38 reveals a new role in actin arrangement.; Authors - Tina Beyer
human	WB	Anal Chem; Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells; Authors - Zi Gao