WDR19/IFT144 Polyclonal antibody

WDR19/IFT144 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13647-1-AP

Synonyms

IFT144, KIAA1638, ORF26, PWDMP, WD repeat domain 19, WDR19, WDR19/IFT144

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Validation Data Gallery

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  WB analysis of HEK-293 using 13647-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13647-1-AP (WDR19/IFT144 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13647-1-AP

  IP result of anti-WDR19/IFT144 (IP:13647-1-AP, 4ug; Detection:13647-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human prostate hyperplasia using 13647-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 13647-1-AP (WDR19/IFT144 antibody) at dilution of 1:50.

• 

  IHC staining of human prostate hyperplasia using 13647-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 13647-1-AP (WDR19/IFT144 antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 13647-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13647-1-AP (WDR19/IFT144 antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 13647-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13647-1-AP (WDR19/IFT144 antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate hyperplasia tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

13647-1-AP targets WDR19/IFT144 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR19/IFT144 fusion protein Ag4599
• Full Name: WD repeat domain 19
• Calculated Molecular Weight: 1342 aa, 152 kDa
• Observed Molecular Weight: 145-152 kDa
• GenBank Accession Number: BC032578
• Gene Symbol: WDR19
• Gene ID (NCBI): 57728
• RRID: AB_10598484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse		Proc Natl Acad Sci U S A; Primary cilia signaling mediates intraocular pressure sensation.; Authors - Na Luo
human		Am J Hum Genet; Patient-iPSC-Derived Kidney Organoids Show Functional Validation of a Ciliopathic Renal Phenotype and Reveal Underlying Pathogenetic Mechanisms.; Authors - Thomas A Forbes
mouse	WB	Elife; A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.; Authors - Tooba Quidwai
mouse	WB	J Cell Physiol; Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.; Authors - Caleb S Hayes
human	WB	PLoS One; Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.; Authors - Yanan Xu
human		J Assist Reprod Genet; A novel homozygous mutation in WDR19 induces disorganization of microtubules in sperm flagella and nonsyndromic asthenoteratospermia.; Authors - Xiaoqing Ni