IFT57 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT57 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 11083-1-AP

Synonyms

HIPPI, HIP1-interacting protein, Estrogen-related receptor beta-like protein 1, ESRRBL1, DERP8

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Validation Data Gallery

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  WB analysis of mouse retina using 11083-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11083-1-AP

  WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells.

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  WB analysis using 11083-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11083-1-AP

  IP result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 11083-1-AP

  Immunofluorescent analysis of MDCK cells using 11083-1-AP (IFT57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse retina tissue, NIH/3T3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IF: See 11 publications below
• IP: See 2 publications below

Product Information

11083-1-AP targets IFT57 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT57 fusion protein Ag1566
• Full Name: intraflagellar transport 57 homolog (Chlamydomonas)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC011899
• Gene Symbol: IFT57
• Gene ID (NCBI): 55081
• RRID: AB_2121957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman
mouse	WB	Dev Cell; The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.; Authors - Gerald M Liew
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
mouse	IF	Proc Natl Acad Sci U S A; Primary cilia signaling mediates intraocular pressure sensation.; Authors - Na Luo