IFT43 Polyclonal antibody

IFT43 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24338-1-AP

Synonyms

C14orf179, Intraflagellar transport protein 43 homolog isoform X2, Intraflagellar transport protein 43 homolog

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Validation Data Gallery

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  IHC staining of human testis using 24338-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24338-1-AP (IFT43 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24338-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24338-1-AP (IFT43 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 24338-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using IFT43 antibody (24338-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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  IF Staining of hTERT-RPE1 using 24338-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT43 antibody (24338-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells, hTERT-RPE1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

24338-1-AP targets IFT43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT43 fusion protein Ag19434
• Full Name: chromosome 14 open reading frame 179
• Calculated Molecular Weight: 213 aa, 24 kDa
• GenBank Accession Number: BC010436
• Gene Symbol: IFT43
• Gene ID (NCBI): 112752
• RRID: AB_2749824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380)

Publications

Species	Application	Title
mouse	WB	Brain; De novo purine biosynthesis is a major driver of chemoresistance in glioblastoma.; Authors - Jack M Shireman
mouse	IF	Proc Natl Acad Sci U S A; Primary cilia signaling mediates intraocular pressure sensation.; Authors - Na Luo
human	IF	Elife; Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.; Authors - Laura Vuolo
mouse		Hum Mol Genet; Ift172 conditional knock-out mice exhibit rapid retinal degeneration and protein trafficking defects.; Authors - Priya R Gupta
mouse	WB	J Cell Physiol; Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.; Authors - Caleb S Hayes