Tested Applications
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24338-1-AP targets IFT43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19434 Product name: Recombinant human C14orf179 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-213 aa of BC010436 Sequence: MEDLLDLDEELRYSLATSRAKMGRRAQQESAQAENHLNGKNSSLTLTGETSSAKLPRCRQGGWAGDSVKASNGTQTGKQQLDLNACYHKTHHRNLGLASLEEADIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLMKYSAIQTLDGEIDLKLLTKVLAPEHEVREDDVGWDWDHLFTEVSSEVLTEWDPLQTEKEDPAGQARHT Predict reactive species |
| Full Name | chromosome 14 open reading frame 179 |
| Calculated Molecular Weight | 213 aa, 24 kDa |
| GenBank Accession Number | BC010436 |
| Gene Symbol | IFT43 |
| Gene ID (NCBI) | 112752 |
| RRID | AB_2749824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IFT43 antibody 24338-1-AP | Download protocol |
| IHC protocol for IFT43 antibody 24338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IFT43 polyclonal antibody (Cat# 24338-1-AP) has 5 total citations. These appear in Brain, PNAS, Journal of Cellular Physiology, Human Molecular Genetics, and eLife. The associated research fields include glioblastoma chemoresistance, primary cilia signaling and intraocular pressure regulation, skeletal muscle atrophy with MAP kinase/Hedgehog signaling, retinal degeneration and protein trafficking defects, and primary cilia formation involving dynein intermediate chains.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Primary cilia signaling mediates intraocular pressure sensation. | ||
J Cell Physiol Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling. | ||
Hum Mol Genet Ift172 conditional knock-out mice exhibit rapid retinal degeneration and protein trafficking defects. | ||
Elife Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function. |
