Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Conjugate

Unconjugated

CloneNo.

240953C9

Cat no : 98136-1-RR

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG

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Validation Data Gallery

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  WB analysis using 98136-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 98136-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  SDS-PAGE experiment of antibody using 98136-1-RR

  Purity of 98136-1-RR (IgG Isotype Control antibody) was determined by SDS- polyacrylamide gel electrophoresis. The antibody was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  IP experiment of HepG2 using 98136-1-RR

  IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

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  IHC staining of human placenta using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 82719-13-RR (PD-L1/CD274 Recombinant antibody) or unstained (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

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  IF Staining of MCF-7 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).PCYT1A Recombinant antibody (83100-1-RR) is a positive control.

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  IF Staining of K-562 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CD71 Recombinant antibody (81793-2-RR) is a positive control.

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  IF Staining of A549 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).TMEM53 Recombinant antibody (83554-3-RR) is a positive control.

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  IF Staining of HeLa using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 98136-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of MCF-7 using 98136-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of RAW 264.7 using 98136-1-RR

  1x10^6 RAW 264.7 cells were intracellularlly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug IGFBP2 Recombinant antibody (84092-3-RR, Clone: 241363D8) and PE-Conjugated Goat Anti-Rabbit IgG (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using 98136-1-RR

  1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

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  FC experiment of rat splenocytes using 98136-1-RR

  1x10^6 rat splenocytes cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) and PE-conjugated Goat Anti-Rabbit IgG, or unstain. Cells were not fixed.

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  FC experiment of HeLa using 98136-1-RR

  1x10^6 HeLa cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Anti-Human MICA Rabbit Recombinant Antibody (98110-1-RR, Clone:240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98136-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or unstain. Cells were not fixed.

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Tested Applications

• Positive IHC detected in: Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG Isotype Control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.