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Anti-Mouse CD40 Rabbit Recombinant Antibody

CD40 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240874G8

Cat no : 98122-1-RR

Synonyms

Tnfrsf5, CD40 antigen, Bp50, 240874G8

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR)

    FC experiment of mouse splenocytes using 98122-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR)

    FC experiment of mouse splenocytes using 98122-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98122-1-RR

    Affinity and Kinetic Characterization of 98122-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98122-1-RR against Mouse CD40 were performed. The affinity constant is 0.997 nM.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98122-1-RR is CD40 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD40 antigen
Calculated Molecular Weight32 kDa
GenBank Accession NumberNM_011611.2
Gene Symbol Cd40
Gene ID (NCBI) 21939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Protocols

Product Specific Protocols
FC protocol for CD40 antibody 98122-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols