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CD71 Recombinant antibody

CD71 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2P14

Cat no : 81793-2-RR

Synonyms

TFR1, TFR, T9, sTfR, p90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD71 Recombinant antibody (81793-2-RR)

    WB analysis using 81793-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Recombinant antibody (81793-2-RR)

    IF Staining of K-562 using 81793-2-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Recombinant antibody (81793-2-RR)

    IF Staining of K-562 using 81793-2-RR

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 81793-2-RR

    Affinity and Kinetic Characterization of 81793-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81793-2-RR against Human CD71 were performed. The affinity constant is 6.63 nM.

Tested Applications

Positive WB detected inPC-12 cells, HT-1080 cells, Raji cells, K-562 cells, HeLa cells
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81793-2-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD71 fusion protein Ag0123
Full Name transferrin receptor (p90, CD71)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 81793-2-RRDownload protocol
IF protocol for CD71 antibody 81793-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols