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TMEM53 Recombinant antibody

TMEM53 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240511C3

Cat no : 83554-3-RR

Synonyms

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    IF Staining of A549 using 83554-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using TMEM53 Recombinant antibody (83554-3-RR)

    FC experiment of A549 using 83554-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83554-3-RR

    Affinity and Kinetic Characterization of 83554-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83554-3-RR is TMEM53 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM53 fusion protein Ag21321
Full Name transmembrane protein 53
Calculated Molecular Weight 277 aa, 32 kDa
GenBank Accession NumberBC064520
Gene Symbol TMEM53
Gene ID (NCBI) 79639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM53 was initially described as a nuclear envelope transmembrane (NET) protein because of its nuclear envelope localization. Research has found that TMEM53 functionality does not rely on viral RNA binding or canonical IFN responses, and TMEM53 exerts an antiviral effect on other bat HKU2-related CoVs in a species-specific manner. Therefore, TMEM53 may be a potential therapeutic target for HKU2-related CoV infections and useful for preparing for future outbreaks of this viral species. (PMID: 37584551)

Protocols

Product Specific Protocols
IF protocol for TMEM53 antibody 83554-3-RRDownload protocol
FC protocol for TMEM53 antibody 83554-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols