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PCYT1A Recombinant antibody

PCYT1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230393B4

Cat no : 83100-1-RR

Synonyms

PCYT1, CTPCT, CT A, CCT alpha, CCT A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCYT1A Recombinant antibody (83100-1-RR)

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCYT1A Recombinant antibody (83100-1-RR)

    WB analysis using 83100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCYT1A Recombinant antibody (83100-1-RR)

    IF Staining of MCF-7 using 83100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using PCYT1A Recombinant antibody (83100-1-RR)

    FC experiment of A431 using 83100-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83100-1-RR

    Affinity and Kinetic Characterization of 83100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, mouse testis tissue, mouse brain tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83100-1-RR targets PCYT1A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCYT1A fusion protein Ag34268
Full Name phosphate cytidylyltransferase 1, choline, alpha
Calculated Molecular Weight42 kDa
Observed Molecular Weight38-41 kDa
GenBank Accession NumberNM_005017
Gene Symbol PCYT1A
Gene ID (NCBI) 5130
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PCYT1A antibody 83100-1-RRDownload protocol
IF protocol for PCYT1A antibody 83100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hua (Verified Customer) (08-30-2024)

Great antibody. Very strong signal and clean background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: Mouse liver
PCYT1A Antibody Western Blot validation (1:10,000 dilution) in Mouse liver (Cat no:83100-1-RR)