Rabbit IgG Isotype Control Recombinant Antibody, PBS Only

IgG Isotype Control Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Conjugate

Unconjugated

CloneNo.

240953C9

Cat no : 98136-1-PBS

Synonyms

Rabbit Isotype Control, Rabbit IgG Isotype Control, Rabbit IgG Control, Rabbit IgG

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Validation Data Gallery

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  WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  SDS-PAGE experiment of antibody using 98136-1-RR (same clone as 98136-1-PBS)

  Purity of 98136-1-RR (IgG Isotype Control antibody) was determined by SDS- polyacrylamide gel electrophoresis. The antibody was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

  IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 98136-1-RR (same clone as 98136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 82719-13-RR (PD-L1/CD274 Recombinant antibody) or unstained (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 98136-1-RR (same clone as 98136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 98136-1-RR (same clone as 98136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 98136-1-RR (same clone as 98136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).PCYT1A Recombinant antibody (83100-1-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 98136-1-RR (same clone as 98136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CD71 Recombinant antibody (81793-2-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 98136-1-RR (same clone as 98136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).TMEM53 Recombinant antibody (83554-3-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of RAW 264.7 using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 RAW 264.7 cells were intracellularlly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug IGFBP2 Recombinant antibody (84092-3-RR, Clone: 241363D8) and PE-Conjugated Goat Anti-Rabbit IgG (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 HeLa cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Anti-Human MICA Rabbit Recombinant Antibody (98110-1-RR, Clone:240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or unstain. Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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  FC experiment of rat splenocytes using 98136-1-RR (same clone as 98136-1-PBS)

  1x10^6 rat splenocytes cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) and PE-conjugated Goat Anti-Rabbit IgG, or unstain. Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

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Product Information

98136-1-PBS targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG Isotype Control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.