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Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

240725C2

Cat no : 98060-1-RR

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR)

    FC experiment of rat splenocytes using 98060-1-RR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) (red) or sotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98060-1-RR

    Affinity and Kinetic Characterization of 98060-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98060-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 0.347 nM.

Tested Applications

Positive FC detected inrat splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98060-1-RR is ICAM-1/CD54 Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name intercellular adhesion molecule 1
Calculated Molecular Weight60 kDa
GenBank Accession NumberNM_012967.1
Gene Symbol Icam1
Gene ID (NCBI) 25464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
FC protocol for ICAM-1/CD54 antibody 98060-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols