CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240953C9

Cat no : CL488-98136

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG Isotype, Rabbit IgG, 240953C9

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using CL488-98136

  1x10^6 human peripheral blood leukocytes were unstained(left, blue in histogram) or surface stained with 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone:240953C9)(mid, red in histogram), or 0.25 ug CoraLite® Plus 488 Anti-Human CD45 Rabbit Recombinant Antibody (CL488-98117, Clone: 241670E10) (right, green in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG Isotype Control
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.