VDAC1/2/3 Polyklonaler Antikörper

VDAC1/2/3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (1)

Kat-Nr. : 11663-1-AP

Synonyme

VDAC2, VDAC-2, VDAC 2, hVDAC2, FLJ23841

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Galerie der Validierungsdaten

• 

  WB analysis using 11663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11663-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate using 11663-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate using 11663-1-AP (VDAC1/2/3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Prostatagewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11663-1-AP bindet in WB, IHC, IF, CoIP, ELISA VDAC1/2/3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VDAC1/2/3 fusion protein Ag2266
• Vollständiger Name: voltage-dependent anion channel 2
• Berechnetes Molekulargewicht: 294 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000165
• Gene symbol: VDAC2
• Gene ID (NCBI): 7417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDACs (Voltage Dependent Anion selective Channels), also known as mitochondrial porins, are a family of pore-forming proteins discovered in the mitochondrial outer membrane. Mammals show a conserved genetic organization of the VDAC genes. It's reported that the amount of VDAC transcripts in liver is usually lower than in the other tissues. VDAC2 and expecially VDAC3 are highly expressed in testis, while mouse VDAC1 is poorly expressed in this tissue.(PMID: 22020053)

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.; Authors - Shintaro Mise
mouse	IP	Autophagy; MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.; Authors - Jia Yuan
human,mouse	WB,IF	Cell Death Differ; SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.; Authors - Ying Zhang
mouse	WB	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
rat	WB,CoIP	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB	EMBO Mol Med; Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1.; Authors - Lin Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jun (Verified Customer) (06-12-2022)

Works very well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

VDAC1/2/3 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11663-1-AP)

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