VDAC2 Polyclonal antibody

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  WB analysis of HepG2 using 55261-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 55261-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55261-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

VDAC2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55261-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

hVDAC2, VDAC 2, VDAC2

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, HuH-7 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55261-1-AP targets VDAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: NM_003375
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• RRID: AB_10852961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45880
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

Publications

Species	Application	Title
mouse	WB	Nature; Gasdermin D-mediated metabolic crosstalk promotes tissue repair; Authors - Zhexu Chi
human	WB	Cell Physiol Biochem; Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.; Authors - Meiying Yang