VDAC2 Polyclonal antibody

VDAC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55261-1-AP

Synonyms

hVDAC2, VDAC 2, VDAC2

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 55261-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55261-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 55261-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 55261-1-AP (VDAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55261-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55261-1-AP (VDAC2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, HuH-7 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55261-1-AP targets VDAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: NM_003375
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• RRID: AB_10852961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC2.

Publications

Species	Application	Title
human	WB	Cell Physiol Biochem; Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress.; Authors - Meiying Yang

Related Products

VDAC2 Monoclonal antibody

66388-1-Ig

VDAC1/2/3 Polyclonal antibody

11663-1-AP

VDAC1/Porin Monoclonal antibody

66345-1-Ig

VDAC1/Porin Polyclonal antibody

55259-1-AP

VDAC1/2 Polyclonal antibody

10866-1-AP

VDAC3 Polyclonal antibody

55260-1-AP

BAK Polyclonal antibody

14673-1-AP

BAX Monoclonal antibody

60267-1-Ig

Cytochrome c Monoclonal antibody

66264-1-Ig

Bcl-XL Monoclonal antibody

66020-1-Ig