Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat no : 66020-1-Ig

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



Tested Applications

Positive WB detected inJurkat cells, A431 cells, HeLa cells, Ramos cells, K-562 cells, Raw 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue, human testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66020-1-Ig is Bcl-XL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IHC protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IF protocol for Bcl-XL antibody 66020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
humanWB

J Immunother Cancer

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells

Authors - Songbo Zhao
humanWB

Blood Cancer J

The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.

Authors - Xinan Qiao
humanWB

Antioxid Redox Signal

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Authors - Tingting Ren
humanWB

J Med Chem

Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.

Authors - Er-Jun Hao
humanWB

Oxid Med Cell Longev

One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence

Authors - Yingjie Qing