Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat







Cat no : 66020-1-Ig


Apoptosis regulator Bcl X, BCL2L1, Bcl-XL

Tested Applications

Positive WB detected inJurkat cells, A431 cells, HeLa cells, Ramos cells, K-562 cells, Raw 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue, human testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66020-1-Ig is Bcl-XL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.


Product Specific Protocols
WB protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IHC protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IF protocol for Bcl-XL antibody 66020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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