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VDAC1/2 Polyclonal antibody

VDAC1/2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10866-1-AP

Synonyms

hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC2


"VDAC1/2 Antibodies" Comparison

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Applications:
  • Western Blot (WB) analysis of various lysates using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis using 10866-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of HeLa using 10866-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of mouse kidney using 10866-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of human heart using 10866-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of mouse brain using 10866-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of DU 145 using 10866-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis of HL-60 using 10866-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis using 10866-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis using 10866-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VDAC1/2 Polyclonal antibody (10866-1-AP)

    WB analysis using 10866-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of human colon using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of human colon using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of mouse heart using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of mouse heart using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of human pancreas using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IHC staining of human pancreas using 10866-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IF Staining of human liver cancer using 10866-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using VDAC1/2 Polyclonal antibody (10866-1-AP)

    IF Staining of human liver cancer using 10866-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).


Tested Applications

Positive WB detected inHEK-293 cells, 4T1 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, mouse brain tissue, mouse kidney tissue, MCF-7 cells, MDA-MB-453s cells, mouse heart tissue, rat heart tissue, Jurkat cells, rat kidney tissue
Positive IHC detected inhuman colon tissue, human pancreas tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10866-1-AP targets VDAC1/2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Pieris rapae, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VDAC1/2 fusion protein Ag1144
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC008482
Gene symbol VDAC1
Gene ID (NCBI) 7416
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.

Protocols

Product Specific Protocols
WB protocol for VDAC1/2 antibody 10866-1-APDownload protocol
IHC protocol for VDAC1/2 antibody 10866-1-APDownload protocol
IF protocol for VDAC1/2 antibody 10866-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseWB

Circ Res

NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.

Authors - Dan Tong
humanWB

Nat Commun

SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.

Authors - Xun Shangguan
humanWB

Am J Hum Genet

CLPB Variants Associated with Autosomal-Recessive Mitochondrial Disorder with Cataract, Neutropenia, Epilepsy, and Methylglutaconic Aciduria.

Authors - Carol Saunders
humanWB

Nucleic Acids Res

Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease.

Authors - Kana Asano

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Macarena (Verified Customer) (10-07-2022)

Good results for both IP and WB

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cell lines
FH

Alberto (Verified Customer) (01-09-2020)

Clear sharp band at the expected molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain
VDAC1/2 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain (Cat no:10866-1-AP)
FH

Wanxia (Verified Customer) (10-22-2019)

Excellent WB results and very low background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500 dilution
  • Cell Tissue Type: Mouse Brain