VDAC1/2 Polyclonal antibody

• Featured Product
• KD/KO Validated

VDAC1/2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Publications

161

Reviews (3)

Cat no : 10866-1-AP

Synonyms

Porin, VDAC1, VDAC1/Porin, Porin 31HM, Porin 31HL

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 10866-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10866-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10866-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10866-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10866-1-AP (VDAC1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 10866-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10866-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10866-1-AP (VDAC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 10866-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10866-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 10866-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 10866-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 10866-1-AP (VDAC1/2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse liver using 10866-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/2 antibody (10866-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10866-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/2 Polyclonal antibody (10866-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, 4T1 cells, Jurkat cells, C6 cells, rat kidney tissue, MCF-7 cells, MDA-MB-453s cells
• Positive IHC detected in: human colon tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue, mouse liver tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:100-1:400
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 150 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• CoIP: See 2 publications below

Product Information

10866-1-AP targets VDAC1/2 in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, zebrafish, pieris rapae
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VDAC1/2 fusion protein Ag1144
• Full Name: voltage-dependent anion channel 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC008482
• Gene Symbol: Porin
• Gene ID (NCBI): 7416
• RRID: AB_2257153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
human	WB	Cell Rep Med; Urolithin A improves muscle strength, exercise performance, and biomarkers of mitochondrial health in a randomized trial in middle-aged adults.; Authors - Anurag Singh
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Acta Pharm Sin B; Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.; Authors - Xin Sun
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena (Verified Customer) (10-07-2022)

Good results for both IP and WB

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cell lines

FH

Alberto (Verified Customer) (01-09-2020)

Clear sharp band at the expected molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain

VDAC1/2 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain (Cat no:10866-1-AP)

FH

Wanxia (Verified Customer) (10-22-2019)

Excellent WB results and very low background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500 dilution
• Cell Tissue Type: Mouse Brain

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