VDAC3 Polyclonal antibody

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• VDAC3 Polyclonal antibody (55260-1-AP)

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• KD/KO Validated

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55260-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

HD VDAC3, hVDAC3, VDAC 3, VDAC-3

• 

  WB analysis of A431 using 55260-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293T using 55260-1-AP

  WB result of VDAC3 antibody (55260-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 55260-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 55260-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55260-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, mouse testis tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55260-1-AP targets VDAC3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: voltage-dependent anion channel 3
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_005662
• Gene Symbol: VDAC3
• Gene ID (NCBI): 7419
• RRID: AB_10973676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer.; Authors - Fang Yang
human	WB	Cancer Discov; BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers; Authors - Guang Lei ; KD Validated
human	WB,IF	Nat Cell Biol; De novo pyrimidine biosynthetic complexes support cancer cell proliferation and ferroptosis defence; Authors - Chuanzhen Yang ; KD Validated
	WB	Nat Commun; VDAC2 enables BAX to mediate apoptosis and limit tumor development.; Authors - Hui San Chin
human		Nat Commun; Cleavage of CAD by caspase-3 determines the cancer cell fate during chemotherapy; Authors - Jingsong Ma
human	WB	Mol Cell; Mitochondrial fumarate inhibits Parkin-mediated mitophagy; Authors - Su Jin Ham