VDAC3 Polyclonal antibody

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  WB analysis of A431 using 55260-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 55260-1-AP

  WB result of VDAC3 antibody (55260-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

• 

  WB analysis of mouse testis using 55260-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 55260-1-AP (VDAC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

VDAC3 Polyclonal Antibody for WB, ELISA

Cat No. 55260-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

55260-1-AP targets VDAC3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 3
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_005662
• Gene Symbol: VDAC3
• Gene ID (NCBI): 7419
• RRID: AB_10973676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y277
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Publications

Species	Application	Title
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang
human	WB	Cell Biosci; The genomic signature of resistance to platinum-containing neoadjuvant therapy based on single-cell data; Authors - Qihai Sui ; KD Validated
human	WB	Ecotoxicol Environ Saf; Lysosomal iron accumulation and subsequent lysosomes-mitochondria iron transmission mediate PFOS-induced hepatocyte ferroptosis; Authors - Jixun Li
human	WB,IP	Front Cell Dev Biol; Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia.; Authors - Ting Zhu
human	WB,IHC,IP	Int J Biol Sci; The lncRNA BDNF-AS/WDR5/FBXW7 axis mediates ferroptosis in gastric cancer peritoneal metastasis by regulating VDAC3 ubiquitination.; Authors - Guoquan Huang
human	WB	Toxins (Basel); Proteomic Studies of the Mechanism of Cytotoxicity, Induced by Palytoxin on HaCaT Cells.; Authors - Dingyuan Cheng