VDAC1/Porin Monoclonal antibody

VDAC1/Porin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E2C7

Cat no : 66345-1-Ig

Synonyms

hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC1/Porin


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  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    WB analysis using 66345-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IHC staining of human heart using 66345-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IHC staining of human heart using 66345-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IHC staining of mouse skeletal muscle using 66345-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IHC staining of mouse skeletal muscle using 66345-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

"VDAC1/Porin Antibodies" Comparison

View side-by-side comparison of VDAC1/Porin antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inMDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW264.7 cells
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66345-1-Ig targets VDAC1/Porin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberNM_003374
Gene symbol VDAC1
Gene ID (NCBI) 7416
RRIDAB_2881725
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

Protocols

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
IHC protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Proc Natl Acad Sci U S A

Ferroptosis as a target for protection against cardiomyopathy.

Authors - Xuexian Fang
humanWB

Free Radic Biol Med

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Authors - Zhiguo Li
mouse

Diabetes

PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.

Authors - Chenrui Li
mouseWB

Oxid Med Cell Longev

The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury.

Authors - Wei Tang
mouseWB

Cell Death Discov

Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice.

Authors - Junqiang Yan
humanWB

Int J Mol Sci

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Authors - Yanqing Li