VDAC1/Porin Polyclonal antibody

• Featured Product
• KD/KO Validated

VDAC1/Porin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA, PLA

Conjugate

Unconjugated

Publications

154

Reviews (1)

Cat no : 55259-1-AP

Synonyms

Porin, VDAC1, Porin 31HM, Porin 31HL, PORIN 31 HL

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of HeLa cells using 55259-1-AP

  WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.

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  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55259-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 55259-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55259-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55259-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 55259-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 55259-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human liver cancer using 55259-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 55259-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human VDAC1/Porin (55259-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

"VDAC1/Porin Antibodies" Comparison

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells
• Positive IHC detected in: human heart tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 142 publications below
• IHC: See 6 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• CoIP: See 4 publications below

Product Information

55259-1-AP targets VDAC1/Porin in WB, IHC, IF-P, FC (Intra), CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, monkey, chicken, bovine, hamster, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_003374
• Gene Symbol: Porin
• Gene ID (NCBI): 7416
• RRID: AB_10837225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Publications

Species	Application	Title
human	WB	Nat Commun; MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration; Authors - Jianxiong Chen
human	WB	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar
monkey	WB	Nat Commun; An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.; Authors - Jingru Wang
human	WB	Nucleic Acids Res; The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.; Authors - Gui-Xin Peng
	IHC	Mol Biol Evol; Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans; Authors - Ming Zhou
mouse	WB	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (04-22-2021)

good antibody. works very well with mouse cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

VDAC1/Porin Antibody Western Blot validation (1:2000 dilution) in (Cat no:55259-1-AP)

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