VDAC2 Monoclonal antibody

VDAC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1D8C11

Cat no : 66388-1-Ig

Synonyms

hVDAC2, VDAC 2, VDAC2

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Validation Data Gallery

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  WB analysis of HEK-293 using 66388-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66388-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66388-1-Ig (VDAC2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66388-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66388-1-Ig (VDAC2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, ROS1728 cells
• Positive IHC detected in: human heart tissue, human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

66388-1-Ig targets VDAC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: voltage-dependent anion channel 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: NM_003375
• Gene Symbol: VDAC2
• Gene ID (NCBI): 7417
• RRID: AB_2881764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publications

Species	Application	Title
human	WB	Drug Dev Res; Xanthatin inhibits non-small cell lung cancer proliferation by breaking the redox balance.; Authors - Yanbo Xie
human,mouse	WB,IHC	Phytomedicine; Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma.; Authors - Fuhao Huang
human	WB	Cancer Discov; BRCA1-mediated dual regulation of ferroptosis exposes a vulnerability to GPX4 and PARP co-inhibition in BRCA1-deficient cancers; Authors - Guang Lei
human	WB	Cell Rep; Cardiolipin oxidized by ROS from complex II acts as a target of gasdermin D to drive mitochondrial pore and heart dysfunction in endotoxemia; Authors - Yan Tang