Tested Applications
| Positive WB detected in | |
| Positive IP detected in | |
| Positive IHC detected in | Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | |
| Positive FC (Intra) detected in | |
| Positive FC detected in | |
| Positive ChIP-qPCR detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP, CoIP, RIP, ELISA, Blocking assay, ChIP-qPCR, CUT&RUN applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Small molecule Predict reactive species |
| Full Name | IgG Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3672282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG Isotype Control antibody 98136-1-RR | Download protocol |
| IF protocol for IgG Isotype Control antibody 98136-1-RR | Download protocol |
| IHC protocol for IgG Isotype Control antibody 98136-1-RR | Download protocol |
| IP protocol for IgG Isotype Control antibody 98136-1-RR | Download protocol |
| WB protocol for IgG Isotype Control antibody 98136-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Rabbit IgG Isotype Control Recombinant Antibody (240953C9, Cat# 98136-1-RR) has 60 citations across journals including Nature Communications, Signal Transduction and Targeted Therapy, Cell Metabolism, Advanced Science, Cell Death & Disease, Free Radical Biology and Medicine, Oncogene, and Science Advances. Research fields span oncology (breast, liver, glioblastoma, renal, ovarian, and lung cancers), immunology, metabolism, neurology, reproductive biology, inflammation, autophagy, and epigenetics.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Family with sequence similarity 114 member A1 orchestrates immune evasion in triple-negative breast cancer | ||
Signal Transduct Target Ther KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma | ||
Cell Metab Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression | ||
Autophagy ATG9 inhibits rickettsia binding to the Host cell surface by blocking the rOmpB-XRCC6/KU70 interaction | ||
Nat Commun Interplay of ECM organization, ROCK signaling, and cell polarity drives mesothelium formation and lung growth. | ||
Nat Commun Dysregulation of GTPase-activating protein-binding protein1 in the pathogenesis of metabolic dysfunction-associated steatotic liver disease. |
Reviews
What customers say
One reviewer (5/5 stars) used this isotype control in flow cytometry on human fibroblasts and reported it works well, calling it a "brilliant isotype control." Overall, the limited feedback is positive, with the sole user confirming reliable performance in FC assays.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dania (Verified Customer) (12-08-2025) | Brilliant isotype control, works well with flow cytometry assays
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