Autophagy Expanded Antibody Kit

Host

Rabbit/Mouse

Reactivity

Target dependent

Applications

Target dependent

Conjugate

Unconjugated

Cat no : PK30005



Product Information

The Autophagy Expanded Antibody Kit provides a cost-effective tool for studying key proteins involved in the autophagy pathway. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.

Kit Components

The Autophagy Expanded Antibody Kit contains antibodies against 10 key protein targets playing critical roles in the autophagy pathway. 

Antigen Catalog No. Host, clonality Tested Reactivity Applications Volume
ULK1 20986-1-AP             Rabbit polyclonal H, M, R WB, IHC, IF, ELISA 20 uL
Beclin 1 66665-1-Ig             Mouse monoclonal H, M, R WB, IP, IHC, IF, ELISA 20 uL
ATG9A 26276-1-AP             Rabbit polyclonal H, M WB, IHC, IF, ELISA 20 uL
LC3 81004-1-RR             Rabbit monoclonal H, M, R, Pg WB, IHC, IF, ELISA 20 uL
p62 80294-1-RR             Rabbit monoclonal H WB, IP, IHC, IF, ELISA 20 uL
ATG5 81803-1-RR             Rabbit monoclonal H, M, R WB, IP, IHC, ELISA 20 uL
ATG16 L1 29445-1-AP             Rabbit polyclonal H, M WB, IHC, IF, ELISA 20 uL
ATG12 11264-1-AP             Rabbit polyclonal H, M WB, IHC, IF, ELISA 20 uL
Ubiquitin 80992-1-RR             Rabbit monoclonal H WB, IHC, IF, ELISA 20 uL
LAMP1 21997-1-AP             Rabbit polyclonal H WB, IP, IHC, FC, ELISA 20 uL

Also see our 'Autophagy Essentials Antibody Kit' on the following page  https://www.ptglab.com/products/Autophagy-Essentials-Antibody-Kit-PK30004.htm

Storage

Store at -20°C. Stable for one year from the date of receipt.

Background Information

Autophagy is a highly dynamic process consisting of the following three steps: (1) autophagosome formation, (2) autophagosome-lysosome fusion, and (3) degradation. It can be induced by multiple signaling pathways related to various triggers including nutrient deprivation, growth factor signaling, and cellular stress. ULK1 and Beclin 1 are critical for the initiation of autophagy. The process of autophagosome formation proceeds through the steps of initiation, nucleation, elongation, closure, and ultimately fusion, each of which is regulated by various ATG proteins. Ubiquitination of various autophagy-related proteins and regulatory proteins are critical for the precise regulation of the autophagy pathway.  

The ideal approach for measuring autophagy is to assess autophagic flux, which represents the rate of degradation of the autophagic pathway. The most widely used method for measuring autophagic flux is to detect the processing of the autophagosomal membrane protein, LC3. Analyzing autophagy substrates such as p62/SQSTM1 is often recommended in addition to measuring LC3-II turnover for accurate assessment of autophagic flux. The fusion of autophagosomes with lysosomes can be monitored by analyzing the autophagosomal marker LC3 and the lysosomal marker, LAMP simultaneously. 

Standard Protocols

Click here to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30005, Autophagy Expanded Antibody Kit, Proteintech, IL, USA