ATG16L1 Polyclonal antibody

ATG16L1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29445-1-AP

Synonyms

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

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Validation Data Gallery

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  WB analysis using 29445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29445-1-AP (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29445-1-AP

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 29445-1-AP (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29445-1-AP (ATG16L1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29445-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29445-1-AP (ATG16L1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 29445-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG16L1 antibody (29445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HSC-T6 cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

29445-1-AP targets ATG16L1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG16L1 fusion protein Ag31318
• Full Name: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 607 aa, 68 kDa
• Observed Molecular Weight: 68-75 kDa
• GenBank Accession Number: BC000061
• Gene Symbol: ATG16L1
• Gene ID (NCBI): 55054
• RRID: AB_2918308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publications

Species	Application	Title
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang
human	WB	Anticancer Drugs; Purvalanol A induces apoptosis and reverses cisplatin resistance in ovarian cancer; Authors - Xiaoyi Zhang